ID   NU4M_METSE              Reviewed;         491 AA.
AC   O47497;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Metridium senile (Brown sea anemone) (Frilled sea anemone).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Nynantheae; Metridiidae; Metridium.
OX   NCBI_TaxID=6116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7966369; DOI=10.1007/bf00160271;
RA   Pont-Kingdon G.A., Beagley C.T., Okimoto R., Wolstenholme D.R.;
RT   "Mitochondrial DNA of the sea anemone, Metridium senile (Cnidaria):
RT   prokaryote-like genes for tRNA(f-Met) and small-subunit ribosomal RNA, and
RT   standard genetic code specificities for AGR and ATA codons.";
RL   J. Mol. Evol. 39:387-399(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=White morph;
RA   Beagley C.T., Okimoto R., Wolstenholme D.R.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; S75445; AAB32499.1; -; Genomic_DNA.
DR   EMBL; AF000023; AAC04640.1; -; Genomic_DNA.
DR   PIR; T11894; T11894.
DR   RefSeq; NP_009263.1; NC_000933.1.
DR   AlphaFoldDB; O47497; -.
DR   SMR; O47497; -.
DR   GeneID; 808779; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..491
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117954"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        372..392
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..432
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        457..477
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   491 AA;  55155 MW;  E1332183648C8118 CRC64;
     MIFHKNWLGL IFLLLFLGII NVMRVPRDNS VKLKRTALEW SLATLTATLI LWAAFDMEGQ
     FQTINQTEWI VPPALNFKWG PLFLAVDGIS LFFLILTALL TPICILISWN SIKFLLKEFL
     LCLLFLEVLL MGVFSALDLL LFYILFEGIL IPMFLLIGVW GSREEKVRAS YYFFFYTFVG
     SVFMLLGIFQ LYSSVGTTDY QALLNIELPI STQKWIFAGF FLSLAVKIPQ VPFHIWLPQA
     HVEAPVSGSV ILAGILLKLG GYGFLRFTWP ILPAATEYFA PFVIMLSVIA IIYGSLTTCR
     QVDLKRLIAY SSVAHMGLVT LGLFTHTIEG LVAAVFMMLA HGLVSSALFI AVTYLYERHH
     TRLIKYYRGI TFSMPIFVSV FLVLTLTNMA IPLSCNFVAE FFSLLAAFEY NIVIGVLAAT
     GMVWSAAYSL YLYNRVSFGA SSNYLLFTRD LNRRELIAIS PLVILIFILG VLPSLIIDPV
     KNAIMFSPGG A