NU4M_MOUSE
ID NU4M_MOUSE Reviewed; 459 AA.
AC P03911;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=Mtnd4; Synonyms=mt-Nd4, Nd4;
OS Mus musculus (Mouse).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7332926; DOI=10.1016/0092-8674(81)90300-7;
RA Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A.;
RT "Sequence and gene organization of mouse mitochondrial DNA.";
RL Cell 26:167-180(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=12954771; DOI=10.1093/nar/gkg739;
RA Bayona-Bafaluy M.P., Acin-Perez R., Mullikin J.C., Park J.S.,
RA Moreno-Loshuertos R., Hu P., Perez-Martos A., Fernandez-Silva P., Bai Y.,
RA Enriquez J.A.;
RT "Revisiting the mouse mitochondrial DNA sequence.";
RL Nucleic Acids Res. 31:5349-5355(2003).
RN [3]
RP PROTEIN SEQUENCE OF 246-255 AND 419-432, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; J01420; AAB48653.1; -; Genomic_DNA.
DR EMBL; V00711; CAA24091.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY172335; AAN85131.1; -; Genomic_DNA.
DR PIR; A00440; QXMS4M.
DR RefSeq; NP_904337.1; NC_005089.1.
DR PDB; 6G2J; EM; 3.30 A; M=1-459.
DR PDB; 6G72; EM; 3.90 A; M=1-459.
DR PDB; 6ZR2; EM; 3.10 A; M=1-459.
DR PDB; 6ZTQ; EM; 3.00 A; M=1-459.
DR PDB; 7AK5; EM; 3.17 A; M=1-459.
DR PDB; 7AK6; EM; 3.82 A; M=1-459.
DR PDB; 7B93; EM; 3.04 A; M=1-459.
DR PDB; 7PSA; EM; 3.40 A; M=1-459.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; P03911; -.
DR SMR; P03911; -.
DR BioGRID; 201550; 4.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; P03911; -.
DR IntAct; P03911; 5.
DR MINT; P03911; -.
DR STRING; 10090.ENSMUSP00000081000; -.
DR PhosphoSitePlus; P03911; -.
DR SwissPalm; P03911; -.
DR jPOST; P03911; -.
DR PaxDb; P03911; -.
DR PeptideAtlas; P03911; -.
DR PRIDE; P03911; -.
DR ProteomicsDB; 293774; -.
DR Antibodypedia; 35362; 24 antibodies from 11 providers.
DR Ensembl; ENSMUST00000082414; ENSMUSP00000081000; ENSMUSG00000064363.
DR GeneID; 17719; -.
DR KEGG; mmu:17719; -.
DR CTD; 4538; -.
DR MGI; MGI:102498; mt-Nd4.
DR VEuPathDB; HostDB:ENSMUSG00000064363; -.
DR eggNOG; KOG4845; Eukaryota.
DR GeneTree; ENSGT00730000111316; -.
DR HOGENOM; CLU_007100_4_0_1; -.
DR InParanoid; P03911; -.
DR OMA; ITRWGNQ; -.
DR OrthoDB; 996580at2759; -.
DR PhylomeDB; P03911; -.
DR TreeFam; TF343520; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR ChiTaRS; mt-Nd4; mouse.
DR PRO; PR:P03911; -.
DR Proteomes; UP000000589; Mitochondrion.
DR RNAct; P03911; protein.
DR Bgee; ENSMUSG00000064363; Expressed in hypothalamus and 60 other tissues.
DR ExpressionAtlas; P03911; baseline and differential.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0007568; P:aging; ISO:MGI.
DR GO; GO:0021549; P:cerebellum development; IEA:Ensembl.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0001701; P:in utero embryonic development; IEA:Ensembl.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl.
DR GO; GO:0035094; P:response to nicotine; IEA:Ensembl.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..459
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117957"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..16
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 20..23
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 24..37
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 38..42
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 53..58
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 62..79
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 80..84
FT /evidence="ECO:0007829|PDB:6G2J"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 89..110
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 115..124
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 126..136
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 142..170
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 176..181
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 190..200
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 203..206
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 210..212
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 215..220
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 226..233
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 234..236
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 237..246
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 247..249
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 253..255
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 259..276
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 282..303
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 306..337
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 341..345
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 355..366
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 370..372
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 373..388
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 390..392
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 393..414
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 416..418
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 431..449
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 451..454
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 459 AA; 51882 MW; 62727CC11CCD137D CRC64;
MLKIILPSLM LLPLTWLSSP KKTWTNVTSY SFLISLTSLT LLWQTDENYK NFSNMFSSDP
LSTPLIILTA WLLPLMLMAS QNHLKKDNNV LQKLYISMLI SLQILLIMTF SATELIMFYI
LFEATLIPTL IIITRWGNQT ERLNAGIYFL FYTLIGSIPL LIALILIQNH VGTLNLMILS
FTTHTLDASW SNNLLWLACM MAFLIKMPLY GVHLWLPKAH VEAPIAGSMI LAAILLKLGS
YGMIRISIIL DPLTKYMAYP FILLSLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIAS
IMIQTPWSFM GATMLMIAHG LTSSLLFCLA NSNYERIHSR TMIMARGLQM VFPLMATWWL
MASLANLALP PSINLMGELF ITMSLFSWSN FTIILMGINI IITGMYSMYM IITTQRGKLT
NHMINLQPSH TRELTLMALH MIPLILLTTS PKLITGLTM
