ID   NU4M_MYXGL              Reviewed;         458 AA.
AC   Q9G2W9; O63920;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Myxine glutinosa (Atlantic hagfish).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Cyclostomata; Myxini;
OC   Myxiniformes; Myxinidae; Myxininae; Myxine.
OX   NCBI_TaxID=7769;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9541532; DOI=10.1007/pl00006317;
RA   Rasmussen A.S., Janke A., Arnason U.;
RT   "The mitochondrial DNA molecule of the hagfish (Myxine glutinosa) and
RT   vertebrate phylogeny.";
RL   J. Mol. Evol. 46:382-388(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11677623; DOI=10.1007/s002390010250;
RA   Delarbre C., Rasmussen A.S., Arnason U., Gachelin G.;
RT   "The complete mitochondrial genome of the hagfish Myxine glutinosa: unique
RT   features of the control region.";
RL   J. Mol. Evol. 53:634-641(2001).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; Y15189; CAA75488.1; -; Genomic_DNA.
DR   EMBL; AJ404477; CAC20658.1; -; Genomic_DNA.
DR   PIR; T13819; T13819.
DR   RefSeq; NP_073282.1; NC_002639.1.
DR   AlphaFoldDB; Q9G2W9; -.
DR   SMR; Q9G2W9; -.
DR   GeneID; 802343; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..458
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117959"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        311..333
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        434..454
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        153
FT                   /note="S -> L (in Ref. 1; CAA75488)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   458 AA;  52033 MW;  06139CCDF69C4F8D CRC64;
     MLKTIFSLIA IIPMTLSFKK QFFWTAFISS SFLMPFLFFI FTPLSFNSSF LMHLQNKLFG
     YDMISAPLVF LSLWMLPLMA LASQHHMKKS PLSYQLLYIT ILIIMQTLLI LTFLSTNLMN
     FYILFESSLI PILLIIFRWG NQKERINAGI YLSFYTLIGS LPLLASLLFL SNTLSTLYIP
     ILMMESSEMY HSPILWLGCF SALLIKTPLY GFHLWLPKAH VEATIAGSMT LAALMLKLGG
     YGMIRLSLMP FLHSPKLSLI LISIALWGAV MTSFICLRQT DLKALIAYSS VSHMGLMTAS
     IMTLSLWGMS GAFIMMIAHG LSSSALFFLA NSNYEKTNTR TIILLRHTQM LLPLTSLWWL
     FIILTNLAMP PSINFISEIT IMSSLFLWSP LTFPFLALTM VITTTYSMSM FMLAQGKLSK
     MTKILSPFST REHLTLFLHL FPMIAIMAYP NMIINIFC