NU4M_ONCMY
ID NU4M_ONCMY Reviewed; 460 AA.
AC P11631;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Oncorhynchus.
OX NCBI_TaxID=8022;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Liver;
RX PubMed=8587139; DOI=10.1007/bf00173174;
RA Zardoya R., Garrido-Pertierra A., Bautista J.M.;
RT "The complete nucleotide sequence of the mitochondrial DNA genome of the
RT rainbow trout, Oncorhynchus mykiss.";
RL J. Mol. Evol. 41:942-951(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 84-262.
RX PubMed=3265651; DOI=10.1007/bf00521273;
RA Davidson W.S., Bartlett S.E., Birt T.P., Green J.M.;
RT "Cloning and sequence analysis of an XbaI fragment of rainbow trout
RT mitochondrial DNA.";
RL Curr. Genet. 14:483-486(1988).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; L29771; AAB03356.1; -; Genomic_DNA.
DR EMBL; X14014; CAA32180.1; -; Genomic_DNA.
DR PIR; T09866; T09866.
DR RefSeq; NP_008299.1; NC_001717.1.
DR AlphaFoldDB; P11631; -.
DR SMR; P11631; -.
DR GeneID; 807978; -.
DR KEGG; omy:807978; -.
DR CTD; 4538; -.
DR OrthoDB; 996580at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..460
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117960"
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 233
FT /note="A -> SS (in Ref. 2; CAA32180)"
FT /evidence="ECO:0000305"
FT CONFLICT 240..254
FT /note="GGYGMMRMMVMLDPL -> EGSHIMCYTSPI (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="A -> V (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="F -> L (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 460 AA; 51497 MW; 6FAE9E3B3B8FE407 CRC64;
MLKILIPTLM LFPTIWLSPA KWLWTTSIAQ SLIIALASLS WLKWSSETGW SSSNLYLATD
PLSTPLLVLT CWLLPLMILA SQSHLSPEPL NRQRAYISLL VSLQTFLVLA FGATEIIMFY
VMFEATLLPT LIIITRWGNQ TERLNAGTYF LFYTLAGSLP LLVALLLMQN DNGTLSMFTL
QYTQPLHLLT WGDKLWWAAC LLAFLVKMPL YGVHLWLPKA HVEAPIAGSM ILAAVLLKLG
GYGMMRMMVM LDPLTKELAY PFIVLALWGI IMTGSICLRQ TDLKSLIAYS SVGHMGLVAG
GILIQTPWGF TGAIILMIAH GLASSALFCL ANTSYERTHS RTMLLARGMQ MILPLMTTWW
FVASLANLAL PPLPNLMGEL MIITSMFNWS YWTLILTGLG TLITASYSLY LFLMTQRGPL
PSHIIALEPT HTREHLLIIL HLIPIVLLIL KPELMWGWCF
