ID   NU4M_PARLI              Reviewed;         464 AA.
AC   P12775;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Paracentrotus lividus (Common sea urchin).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Echinidae; Paracentrotus.
OX   NCBI_TaxID=7656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2544576; DOI=10.1016/s0021-9258(18)60413-2;
RA   Cantatore P., Roberti M., Rainaldi G., Gadaleta M.N., Saccone C.;
RT   "The complete nucleotide sequence, gene organization, and genetic code of
RT   the mitochondrial genome of Paracentrotus lividus.";
RL   J. Biol. Chem. 264:10965-10975(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 172-226 AND 231-250.
RX   PubMed=3596250; DOI=10.1016/0378-1119(87)90091-6;
RA   Cantatore P., Roberti M., Morisco P., Rainaldi G., Gadaleta M.N.,
RA   Saccone C.;
RT   "A novel gene order in the Paracentrotus lividus mitochondrial genome.";
RL   Gene 53:41-54(1987).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; J04815; AAA68142.2; -; Genomic_DNA.
DR   EMBL; M16525; AAA31996.2; -; Genomic_DNA.
DR   EMBL; M16526; AAA31997.1; -; Genomic_DNA.
DR   PIR; A34285; A34285.
DR   RefSeq; NP_008130.2; NC_001572.1.
DR   AlphaFoldDB; P12775; -.
DR   SMR; P12775; -.
DR   GeneID; 807716; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..464
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117964"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        314..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        355..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        389..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   464 AA;  50810 MW;  E5ABCDBD4E608B45 CRC64;
     MMITLILFTT GIVMTTLVIP QNKLWANAIS QSALLSLSSP LLIFSNHWSA PWHNLSNILA
     SDSISAPLIA LSCWLAPISL IASNNTISKQ SELNQRTFII IVIFIAGALI ITFSALELLL
     FYVAFETTLI PTLILITRWG AQMERFQAGL YFMFYTLFGS LPLLISLIAI YISSSSLSIP
     NVELIWTPSE TMETLNIWWA LSIIAFLIKM PIYGFHLWLP KAHVEAPVAG SMILAAILLK
     LGGYGLIRLI NLFATISLNS LSLALITFCS WGALVTSIIC VRQTDLKALI AYSSVGHMSI
     VAAAIFSSTN WGTNGALILM IAYGLVSSDL FSLANTVYER SGTRTLAITR GLKTILPLST
     LWWLVMSAAN LGLPPSPNLI GEILILSSLI AWSIWLFPII GLATIFGAIY SLMIFQLSQQ
     GTPTNNINNI SLSFSREHLL ATLHTLPLIL IIINPISALI TWLK