ID   NU4M_PARTE              Reviewed;         474 AA.
AC   P15581;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4; Synonyms=NDH4;
OS   Paramecium tetraurelia.
OG   Mitochondrion.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX   NCBI_TaxID=5888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Stock 51;
RX   PubMed=2308823; DOI=10.1093/nar/18.1.173;
RA   Pritchard A.E., Seilhamer J.J., Mahalingam R., Sable C.L., Venuti S.E.,
RA   Cummings D.J.;
RT   "Nucleotide sequence of the mitochondrial genome of Paramecium.";
RL   Nucleic Acids Res. 18:173-180(1990).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; X15917; CAA34033.1; -; Genomic_DNA.
DR   PIR; S07754; S07754.
DR   AlphaFoldDB; P15581; -.
DR   SMR; P15581; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..474
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117965"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        454..474
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   474 AA;  54190 MW;  AA93FEFF3CC06CBD CRC64;
     MFAVYLVFSF KKPEASKADL DFFMLTFKYI LKGSFFLMLV LALFCALFTF DLMFSAKNLL
     YPNEYIWDSG DFFFYKNGAL KFSLNLYGLI LVFLCLLTGF VAISTVDNLY SEDKLKFYLI
     FFQFFLAVLG FIKCSDLIAF FFFYEVLMLG SVLVVFFGSY SKKSIHAVIY FVAWTQLGSL
     FVLLACLYIY SLTNSTNFFV IKTFVFSKTQ AMTIYSLLFV GFGIKFPIWP LHYWLTKTHV
     EASTGFSIYL SGFLVKTALF GFYRLTNLIQ VELDTTFFLA VLVAGVIDSS LNMWSQTDLK
     KLVAYCTIQE MNLIAIFFLK GDSSLIAYGF LFTIMHALMS TLMFFLVECI YSRYKSRSTL
     VVNGVFFSFN NLALAIIFMV LFFSGILGTL KFVCEFFVFN LTLHVSWPIG VIFVVVVSAI
     GLIGFSKNWF NAIFCAPSKD VGPDALDLSK KELYIIFLCF AGLIFLTFLP FLMI