NU4M_PIG
ID NU4M_PIG Reviewed; 459 AA.
AC O79881; Q9TDR2;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS Sus scrofa (Pig).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9732457; DOI=10.1007/pl00006388;
RA Ursing B.M., Arnason U.;
RT "The complete mitochondrial DNA sequence of the pig (Sus scrofa).";
RL J. Mol. Evol. 47:302-306(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Landrace;
RX PubMed=10433971; DOI=10.1016/s0378-1119(99)00247-4;
RA Lin C.S., Sun Y.L., Liu C.Y., Yang P.C., Chang L.C., Cheng I.C.,
RA Mao S.J.T., Huang M.C.;
RT "Complete nucleotide sequence of pig (Sus scrofa) mitochondrial genome and
RT dating evolutionary divergence within artiodactyla.";
RL Gene 236:107-114(1999).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AJ002189; CAA05237.1; -; Genomic_DNA.
DR EMBL; AF034253; AAD34194.1; -; Genomic_DNA.
DR PIR; T10981; T10981.
DR RefSeq; NP_008643.1; NC_000845.1.
DR PDB; 5GPN; EM; 5.40 A; h=1-459.
DR PDB; 5GUP; EM; 4.00 A; q=1-459.
DR PDBsum; 5GPN; -.
DR PDBsum; 5GUP; -.
DR AlphaFoldDB; O79881; -.
DR SMR; O79881; -.
DR STRING; 9823.ENSSSCP00000019144; -.
DR PaxDb; O79881; -.
DR PeptideAtlas; O79881; -.
DR PRIDE; O79881; -.
DR Ensembl; ENSSSCT00000019682; ENSSSCP00000019144; ENSSSCG00000018087.
DR Ensembl; ENSSSCT00070061684; ENSSSCP00070052587; ENSSSCG00070030647.
DR GeneID; 808510; -.
DR KEGG; ssc:808510; -.
DR CTD; 4538; -.
DR VGNC; VGNC:99795; MT-ND4.
DR eggNOG; KOG4845; Eukaryota.
DR GeneTree; ENSGT00730000111316; -.
DR HOGENOM; CLU_007100_4_0_1; -.
DR InParanoid; O79881; -.
DR OMA; ITRWGNQ; -.
DR OrthoDB; 996580at2759; -.
DR TreeFam; TF343520; -.
DR Reactome; R-SSC-611105; Respiratory electron transport.
DR Reactome; R-SSC-6799198; Complex I biogenesis.
DR ChiTaRS; ND4; pig.
DR Proteomes; UP000008227; Mitochondrion.
DR Proteomes; UP000314985; Mitochondrion.
DR Bgee; ENSSSCG00000018087; Expressed in prefrontal cortex and 44 other tissues.
DR Genevisible; O79881; SS.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IEA:Ensembl.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..459
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117970"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 361
FT /note="M -> V (in Ref. 1; CAA05237)"
FT /evidence="ECO:0000305"
FT CONFLICT 428
FT /note="A -> P (in Ref. 1; CAA05237)"
FT /evidence="ECO:0000305"
FT CONFLICT 459
FT /note="Y -> YC (in Ref. 1; CAA05237)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 51825 MW; 7625EF89388E92AF CRC64;
MLKIIIPTTM LLPMTWMSKH NMIWINATVH SLLISLISLS LLNQLGENSL NFSLTFFSDS
LSAPLLVLTT WLLPLMLMAS QSHLSKETTT RKKLYITMLI LLQLFLIMTF TATELILFYI
LFEATLVPTL IIITRWGNQT ERLNAGLYFL FYTLAGSLPL LVALVYIQNT TGSLNFLIIH
YWSHPLSNSW SNIFMWLACI MAFMVKMPLY GLHLWLPKAH VEAPIAGSMV LAAVLLKLGG
YGMMRITTIL NPLTNYMAYP FLMLSMWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIVA
IMIQTPWSFM GATALMIAHG LTSSMLFCLA NTNYERVHSR TMILARGLQT LLPLMATWWL
MASLTNLALP PSINLIGELF IITASFSWSN ITIILMGMNM MITALYSLYM LITTQRGKYT
HHINNIKASF TRENALMALH ILPLLLLTLN PKMILGPLY
