ID   NU4M_PISOC              Reviewed;         460 AA.
AC   P24998;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 3.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=NAD4; Synonyms=ND4;
OS   Pisaster ochraceus (Ochre sea star) (Asterias ochracea).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Asterozoa; Asteroidea;
OC   Forcipulatacea; Forcipulatida; Asteriidae; Pisaster.
OX   NCBI_TaxID=7612;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith M.J.;
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31 AND 442-460.
RX   PubMed=1976816; DOI=10.1007/bf02109496;
RA   Smith M.J., Banfield D.K., Doteval K., Gorski S., Kowbel D.J.;
RT   "Nucleotide sequence of nine protein-coding genes and 22 tRNAs in the
RT   mitochondrial DNA of the sea star Pisaster ochraceus.";
RL   J. Mol. Evol. 31:195-204(1990).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; X55514; CAA39131.2; -; Genomic_DNA.
DR   EMBL; X55516; CAA39132.2; -; Genomic_DNA.
DR   PIR; S14212; S14212.
DR   AlphaFoldDB; P24998; -.
DR   SMR; P24998; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..460
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117971"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        300..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   460 AA;  51196 MW;  2E8DCABB3A17D3E4 CRC64;
     MIYLFSLSLS AIFLPWIISN KLYRSISIIN AFIITFFALN VLNNHFFSLW HNISLNFGID
     NLSAPLIILS TWLIPISLLA SINSTNPYTP NSQRTFISTI FFILLALLLT FSALELSLFF
     IAFESTLLPT LVLITRWGAQ KERFQAGIYF IFYTLAGSLP LLIAIISLYN INSSLILPSI
     YLSTNILSFS SATLSIWWIF CFIAFIIKMP IYGFHLWLPK AHVEAPIAGS MILAAILLKL
     GGYGLARTTL IFFFISANYI SFPLITFCIW GALITSIICI RQTDLKALIA YSSVGHMSMV
     AAGIFTLSPW GINGATTLMI AHGLISSSLF CLANILYERN NTRTLAITRG FKLITPLLSL
     WWIITCVTNL GLPPTPNLIG ELLIISSVIN WNISSFIIIT SATVFGAIYS LLIFQNTNSS
     TFPSFISNIQ PISSSEHILL FLHIFPLILI IINPSILLLT