NU4M_PODAN
ID NU4M_PODAN Reviewed; 519 AA.
AC P15582;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=ND4;
OS Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)
OS (Pleurage anserina).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Podosporaceae; Podospora;
OC Podospora anserina.
OX NCBI_TaxID=515849;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A, and s;
RX PubMed=2975708; DOI=10.1016/0022-2836(88)90044-7;
RA Cummings D.J., Domenico J.M.;
RT "Sequence analysis of mitochondrial DNA from Podospora anserina.
RT Pervasiveness of a class I intron in three separate genes.";
RL J. Mol. Biol. 204:815-839(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=s;
RX PubMed=2357736; DOI=10.1007/bf00334517;
RA Cummings D.J., McNally K.L., Domenico J.M., Matsuura E.T.;
RT "The complete DNA sequence of the mitochondrial genome of Podospora
RT anserina.";
RL Curr. Genet. 17:375-402(1990).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; X55026; CAA38806.1; -; Genomic_DNA.
DR EMBL; X14484; CAA32645.1; -; Genomic_DNA.
DR PIR; S02153; S02153.
DR RefSeq; NP_074953.1; NC_001329.3.
DR AlphaFoldDB; P15582; -.
DR SMR; P15582; -.
DR STRING; 515849.P15582; -.
DR TCDB; 3.D.1.6.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR GeneID; 802423; -.
DR KEGG; pan:PoanfMp46; -.
DR Proteomes; UP000001197; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..519
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117972"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 519 AA; 57611 MW; 628C0BEC0DBDF86B CRC64;
MSGLLYALLI IPMIGIFFIL SFDSYNFNIT SNNSNSGSFS EAGAGKNSGG ELLKVLVINN
ELNFYKKIAF ITTIMNLIVS LIIYILFDFS TNQFQFVQDA LELSVYNIYL GVDGLSIYFV
LLTTIIMPIA LISNWNSITH NIKAYLIIIL LLETLLLAVF LVLDVLLFYI FFESILPPLF
ILIGLFGSSN KVRASFYIFL YTLLGSLFLL LSILTMSSLI GTTYLDVLSK SNFEYTTQIF
LFLGVFIAFA VKTPTVFLNS WLLKAHVESP LGGSIVLAGI VLKLSLYGIF RLILPLLPKI
SLNYTSIIFS IGIITIIYAS FSTLRTIDIK ELIAYSSVSH AAVYLIGVFS NIIQGIEGGI
LLGLGHGFVS SGLFICAGGV LYDRSGTRII SYYRGTAQVM PLFSILFFIL CLGNCGAPLT
LNFVGEFMSL YGTFERLPLL GLFSSSSMIF SAAYTIYMYN RIAFGGSFSK FFEENIGDVT
KREFFLLFTL IIFTIMFGIY PSFILDGLHY NVTSLLFGV
