NU4M_PONPY
ID NU4M_PONPY Reviewed; 459 AA.
AC P03908; Q9T9X4;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS Pongo pygmaeus (Bornean orangutan).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9600;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7530363; DOI=10.1073/pnas.92.2.532;
RA Horai S., Hayasaka K., Kondo R., Tsugane K., Takahata N.;
RT "Recent African origin of modern humans revealed by complete sequences of
RT hominoid mitochondrial DNAs.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 308-459.
RX PubMed=6284948; DOI=10.1007/bf01734101;
RA Brown W.M., Prager E.M., Wang A., Wilson A.C.;
RT "Mitochondrial DNA sequences of primates: tempo and mode of evolution.";
RL J. Mol. Evol. 18:225-239(1982).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; D38115; BAA85291.1; -; Genomic_DNA.
DR EMBL; V00675; CAB51802.1; ALT_TERM; Genomic_DNA.
DR PIR; A00437; A00437.
DR RefSeq; NP_008234.1; NC_001646.1.
DR AlphaFoldDB; P03908; -.
DR SMR; P03908; -.
DR GeneID; 807912; -.
DR CTD; 4538; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..459
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117975"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 404
FT /note="A -> T (in Ref. 2; CAB51802)"
FT /evidence="ECO:0000305"
FT CONFLICT 412
FT /note="I -> T (in Ref. 2; CAB51802)"
FT /evidence="ECO:0000305"
FT CONFLICT 420
FT /note="S -> T (in Ref. 2; CAB51802)"
FT /evidence="ECO:0000305"
FT CONFLICT 430
FT /note="S -> F (in Ref. 2; CAB51802)"
FT /evidence="ECO:0000305"
FT CONFLICT 442
FT /note="F -> S (in Ref. 2; CAB51802)"
FT /evidence="ECO:0000305"
FT CONFLICT 457..458
FT /note="LT -> FA (in Ref. 2; CAB51802)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 51508 MW; 0D679A08E6C759CD CRC64;
MLKLIIPTVM LLPLTWLSKT HMIWINTTTH SLIISFIPLL FLNQTNSNLS SYSLLFSSDP
LSTPLLMLTT WLLPLMIMAS QHHLSNEPPS RKKLYLSMLI SLQISLIMTF TATELIMFYI
LFETTLIPTL IIITRWGNQP ERLNAGTYFL FYTLVGSLPL LIALTHTHNT LGSLNIMLLT
LTARELTDSW SNSLMWLAYT MAFMMKMPLY GVHLWLPKAH VEAPIAGSMV LAAVLLKLGG
YGMMRLTIIL DPLTKHMAYP FLVLSLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVVAA
ILIQTPWSFT GATTLMIAHG LTSSLLFCLA NSNYERTHSR IMILSQGLQT LLPLMALWWL
LASLTNLALP PTINLLGELS VLMAMFSWSN ITILLTGLNM LITALYSLYM FITTQRGTPS
HHINNMKPSS TRENTLMLMH LFPILLLSLN PSIIAGLTY
