NU4M_SCYCA
ID NU4M_SCYCA Reviewed; 460 AA.
AC O79410;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MTND4; Synonyms=NAD4, NADH4, ND4;
OS Scyliorhinus canicula (Small-spotted catshark) (Squalus canicula).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC Elasmobranchii; Galeomorphii; Galeoidea; Carcharhiniformes; Scyliorhinidae;
OC Scyliorhinus.
OX NCBI_TaxID=7830;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Muscle;
RX PubMed=9725850; DOI=10.1093/genetics/150.1.331;
RA Delarbre C., Spruyt N., Delmarre C., Gallut C., Barriel V., Janvier P.,
RA Laudet V., Gachelin G.;
RT "The complete nucleotide sequence of the mitochondrial DNA of the dogfish,
RT Scyliorhinus canicula.";
RL Genetics 150:331-344(1998).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; Y16067; CAA76028.1; -; Genomic_DNA.
DR PIR; T11309; T11309.
DR RefSeq; NP_007623.1; NC_001950.1.
DR AlphaFoldDB; O79410; -.
DR SMR; O79410; -.
DR GeneID; 808293; -.
DR CTD; 4538; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..460
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117985"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 460 AA; 52149 MW; 44E7A8A528AFE366 CRC64;
MLKILIPTIM LFPTTWFMNK KWLWSSITTH SLLISLLSLS WFKWNMDIGW DFSNQYLAID
PLSAPLLILT CWLLPLMILA SQNHITPEPL TRQRIYISLL ISLQVFLIMA FSATEMILFY
IMFEATLIPT LIIITRWGNQ TERLNAGTYF LFYTLIGSLP LLIALLFMQN DLNTLSMFII
QYSHLPNPSS WANKFWWTAC LIAFLVKMPL YGVHLWLPKA HVEAPIAGSM ILAAVLLKLG
GYGMMRIIIM LNPITKEMAY PFIILAIWGI VMTSSICLRQ TDLKSMIAYS SVSHMGLVAG
AILIQTPWSF AGAITLMIAH GLVSSALFCL ANTNYERIHS RTLLLARGVQ VILPLMATWW
LLANLANLAL PPSPNLVGEL LIISSLFNWS NWTILLTGIG VLITASYSLY MFLMTQRGLT
SKHLMNLNPS HTREHLLLTL HVLPVLLLIL KPELIWGWTF
