NU4M_SHEEP
ID NU4M_SHEEP Reviewed; 459 AA.
AC O78755;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS Ovis aries (Sheep).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Merinolandschaf {ECO:0000312|Proteomes:UP000002356}; TISSUE=Liver;
RX PubMed=9767689; DOI=10.1007/pl00006401;
RA Hiendleder S., Lewalski H., Wassmuth R., Janke A.;
RT "The complete mitochondrial DNA sequence of the domestic sheep (Ovis aries)
RT and comparison with the other major ovine haplotype.";
RL J. Mol. Evol. 47:441-448(1998).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AF010406; AAD10104.1; -; Genomic_DNA.
DR PIR; T11059; T11059.
DR RefSeq; NP_008415.1; NC_001941.1.
DR PDB; 5LNK; EM; 3.90 A; M=1-459.
DR PDB; 6Q9B; EM; 3.90 A; D4=1-459.
DR PDB; 6QA9; EM; 4.10 A; D4=1-459.
DR PDB; 6QBX; EM; 4.20 A; D4=1-459.
DR PDB; 6QC2; EM; 4.20 A; D4=1-459.
DR PDB; 6QC3; EM; 4.20 A; D4=1-459.
DR PDB; 6QC4; EM; 4.60 A; D4=1-459.
DR PDB; 6QC5; EM; 4.30 A; D4=1-459.
DR PDB; 6QC6; EM; 4.10 A; D4=1-459.
DR PDB; 6QC7; EM; 4.40 A; D4=1-459.
DR PDB; 6QC8; EM; 4.20 A; D4=1-459.
DR PDB; 6QC9; EM; 5.70 A; D4=1-459.
DR PDB; 6QCA; EM; 6.20 A; D4=1-459.
DR PDB; 6QCF; EM; 6.50 A; D4=1-459.
DR PDB; 6ZKA; EM; 2.50 A; M=1-459.
DR PDB; 6ZKB; EM; 2.90 A; M=1-459.
DR PDB; 6ZKC; EM; 3.10 A; M=1-459.
DR PDB; 6ZKD; EM; 2.70 A; M=1-459.
DR PDB; 6ZKE; EM; 2.60 A; M=1-459.
DR PDB; 6ZKF; EM; 2.80 A; M=1-459.
DR PDB; 6ZKG; EM; 3.40 A; M=1-459.
DR PDB; 6ZKH; EM; 3.00 A; M=1-459.
DR PDB; 6ZKI; EM; 2.80 A; M=1-459.
DR PDB; 6ZKJ; EM; 3.00 A; M=1-459.
DR PDB; 6ZKK; EM; 3.70 A; M=1-459.
DR PDB; 6ZKL; EM; 3.10 A; M=1-459.
DR PDB; 6ZKM; EM; 2.80 A; M=1-459.
DR PDB; 6ZKN; EM; 2.90 A; M=1-459.
DR PDB; 6ZKO; EM; 3.80 A; M=1-459.
DR PDB; 6ZKP; EM; 3.20 A; M=1-459.
DR PDB; 6ZKQ; EM; 3.30 A; M=1-459.
DR PDB; 6ZKR; EM; 3.50 A; M=1-459.
DR PDB; 6ZKS; EM; 3.10 A; M=1-459.
DR PDB; 6ZKT; EM; 2.80 A; M=1-459.
DR PDB; 6ZKU; EM; 3.00 A; M=1-459.
DR PDB; 6ZKV; EM; 2.90 A; M=1-459.
DR PDBsum; 5LNK; -.
DR PDBsum; 6Q9B; -.
DR PDBsum; 6QA9; -.
DR PDBsum; 6QBX; -.
DR PDBsum; 6QC2; -.
DR PDBsum; 6QC3; -.
DR PDBsum; 6QC4; -.
DR PDBsum; 6QC5; -.
DR PDBsum; 6QC6; -.
DR PDBsum; 6QC7; -.
DR PDBsum; 6QC8; -.
DR PDBsum; 6QC9; -.
DR PDBsum; 6QCA; -.
DR PDBsum; 6QCF; -.
DR PDBsum; 6ZKA; -.
DR PDBsum; 6ZKB; -.
DR PDBsum; 6ZKC; -.
DR PDBsum; 6ZKD; -.
DR PDBsum; 6ZKE; -.
DR PDBsum; 6ZKF; -.
DR PDBsum; 6ZKG; -.
DR PDBsum; 6ZKH; -.
DR PDBsum; 6ZKI; -.
DR PDBsum; 6ZKJ; -.
DR PDBsum; 6ZKK; -.
DR PDBsum; 6ZKL; -.
DR PDBsum; 6ZKM; -.
DR PDBsum; 6ZKN; -.
DR PDBsum; 6ZKO; -.
DR PDBsum; 6ZKP; -.
DR PDBsum; 6ZKQ; -.
DR PDBsum; 6ZKR; -.
DR PDBsum; 6ZKS; -.
DR PDBsum; 6ZKT; -.
DR PDBsum; 6ZKU; -.
DR PDBsum; 6ZKV; -.
DR AlphaFoldDB; O78755; -.
DR SMR; O78755; -.
DR STRING; 9940.ENSOARP00000000010; -.
DR Ensembl; ENSOART00000000028; ENSOARP00000000010; ENSOARG00000000028.
DR Ensembl; ENSOART00020000027; ENSOARP00020000009; ENSOARG00020000027.
DR GeneID; 808257; -.
DR KEGG; oas:808257; -.
DR CTD; 4538; -.
DR eggNOG; KOG4845; Eukaryota.
DR HOGENOM; CLU_007100_4_0_1; -.
DR OMA; ITRWGNQ; -.
DR OrthoDB; 996580at2759; -.
DR Proteomes; UP000002356; Mitochondrion.
DR Bgee; ENSOARG00000000028; Expressed in cardiac muscle tissue of left auricle and 55 other tissues.
DR ExpressionAtlas; O78755; baseline.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IEA:Ensembl.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..459
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117986"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..16
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 20..22
FT /evidence="ECO:0007829|PDB:6ZKE"
FT HELIX 23..37
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 38..42
FT /evidence="ECO:0007829|PDB:6ZKA"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:6ZKA"
FT STRAND 53..58
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 62..80
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 81..86
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 89..110
FT /evidence="ECO:0007829|PDB:6ZKA"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 115..124
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 126..136
FT /evidence="ECO:0007829|PDB:6ZKA"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 142..171
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 176..182
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 190..206
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 210..212
FT /evidence="ECO:0007829|PDB:6ZKC"
FT HELIX 213..215
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 216..222
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 225..233
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 235..246
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 247..251
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 254..277
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 282..303
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 306..337
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 342..344
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 348..351
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 353..366
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 373..388
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 392..415
FT /evidence="ECO:0007829|PDB:6ZKA"
FT STRAND 416..418
FT /evidence="ECO:0007829|PDB:6ZKE"
FT STRAND 421..423
FT /evidence="ECO:0007829|PDB:6ZKE"
FT HELIX 431..446
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 447..449
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 451..454
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 456..458
FT /evidence="ECO:0007829|PDB:6ZKI"
SQ SEQUENCE 459 AA; 52026 MW; 899F37F114A96C71 CRC64;
MLKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS
LSTPLLILTM WLLPLMLMAS QHHLSKENLA RKKLFISMLI LLQLFLIMTF TATELIFFYI
MFEATLVPTL IIITRWGNQT ERLNAGLYFL FYTLAGSLPL LVALIYIQNT MGSLNFLILQ
YWVQPMPNSW SNTFMWLACM MAFMVKMPLY GLHLWLPKAH VEAPIAGSMV LAAILLKLGG
YGMMRITLLL NPITDFMAYP FIMLSLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIVA
ILIQTPWSYM GATALMIAHG LTSSMLFCLA NSNYERVHSR TMILARGLQT LLPLMAAWWL
LASLTNLALP PSINLIGELF VVMSTFSWSN ITIILMGLNM VITALYSLYM LITTQRGKHT
HHINNILPSF TRENALMSLH MLPLLLLSLN PKIILGPLY
