ID   NU4M_SQUAC              Reviewed;         460 AA.
AC   Q9ZZ45;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Squalus acanthias (Spiny dogfish).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC   Elasmobranchii; Squalomorphii; Squaliformes; Squalidae; Squalus.
OX   NCBI_TaxID=7797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9873084; DOI=10.1007/pl00006439;
RA   Rasmussen A.S., Arnason U.;
RT   "Phylogenetic studies of complete mitochondrial DNA molecules place
RT   cartilaginous fishes within the tree of bony fishes.";
RL   J. Mol. Evol. 48:118-123(1999).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; Y18134; CAA77058.1; -; Genomic_DNA.
DR   PIR; T11543; T11543.
DR   RefSeq; NP_008532.1; NC_002012.1.
DR   AlphaFoldDB; Q9ZZ45; -.
DR   SMR; Q9ZZ45; -.
DR   GeneID; 808384; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..460
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117989"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        310..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        351..371
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   460 AA;  52012 MW;  DC8F549ED04EBE26 CRC64;
     MLKILVPTIM LFLVTWSSPK KWLWPITTTH SLLIALLSLL WFKWNMDMGW DFSNHYLAID
     PLSAPLLILT CWLLPLMILA SQNHISTEPI IRQRIYITLL ISLQTFLIMA FSATELIMFY
     IMFEATLIPT LIIITRWGNQ TERLNAGTYF LFYTLIGSLP LLVALLLMQN DLGSLSMIII
     QYPQPLSLST WADKFWWTAC LIAFLVKMPL YGVHLWLPKA HVEAPIAGSM ILAAVLLKLG
     GYGMMRIIVM LNPLTKEMAY PFLILAIWGV IMTSSICLRQ TDLKSLIAYS SVSHMGLVAG
     AIMIQTPWSF AGAITLMIAH GLVSSALFCL ANTNYERTHS RTLLLARGIQ VMLPLMATWW
     FIANLANLAL PPTPNLMGEL LIISSLFNWS SWTILLTGLG VLITASYSLY MFLMTQRGPA
     SQHLLSLNPS YTREHLLLNL HLIPMLLLIL KPELIWGWTF