ID   NU4M_STRCA              Reviewed;         458 AA.
AC   O21406;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Struthio camelus (Common ostrich).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Palaeognathae; Struthioniformes; Struthionidae;
OC   Struthio.
OX   NCBI_TaxID=8801;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9214748; DOI=10.1093/oxfordjournals.molbev.a025815;
RA   Harlid A., Janke A., Arnason U.;
RT   "The mtDNA sequence of the ostrich and the divergence between paleognathous
RT   and neognathous birds.";
RL   Mol. Biol. Evol. 14:754-761(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11370967; DOI=10.1098/rspb.2001.1587;
RA   Haddrath O., Baker A.J.;
RT   "Complete mitochondrial DNA genome sequences of extinct birds: ratite
RT   phylogenetics and the vicariance biogeography hypothesis.";
RL   Proc. R. Soc. B 268:939-945(2001).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y12025; CAA72753.1; -; Genomic_DNA.
DR   EMBL; AF338715; AAK53354.1; -; Genomic_DNA.
DR   PIR; B99613; B99613.
DR   PIR; T11528; T11528.
DR   RefSeq; NP_115450.1; NC_002785.1.
DR   AlphaFoldDB; O21406; -.
DR   SMR; O21406; -.
DR   GeneID; 803272; -.
DR   CTD; 4538; -.
DR   OrthoDB; 996580at2759; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..458
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117990"
FT   TRANSMEM        21..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        341..361
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   458 AA;  50955 MW;  575118B6297A18AC CRC64;
     MLKIIIPTIM LLPTALLSPK ASLWTNTTTY SLLIATISLQ WLNPTYFPHK NMTPWTGIDQ
     ISAPLLVLSC WLLPLMLLAS QNHLQQEPLV RKRTFIVTLT TIQPFIILAF SATELTLFYI
     SFEATLIPTL ILITRWGNQP ERLSAGIYLL FYTLISSLPL LVTILHLHTQ IGTLHMPTLE
     LTHPLLTHSW TGTLSGLALL MAFMVKAPLY GLHLWLPKAH VEAPIAGSML LAALLLKLGG
     YGIMRVTLLM GPLTDRLSYP FLALALWGAL MTSSICLRQT DLKSLIAYSS VSHMGLVIAA
     SMIQTDWSFS GAMILMISHG LTSSMLFCLA NTNYERTHSR ILLLTRGLQP LLPLMSVWWL
     LANLTNMALP PTTNLMAELT IMIALFNWST PTIILTGLAT LLTASYTLFM LSMTQRGTLP
     THLTSIHNSN TREHLLMTLH IFPMLLLMLK PELISGVI