ID   NU4M_TACAC              Reviewed;         459 AA.
AC   Q8W9G4;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Tachyglossus aculeatus aculeatus (Southeast Australian short-beaked
OS   echidna).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Monotremata; Tachyglossidae; Tachyglossus.
OX   NCBI_TaxID=49271;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Liver;
RX   PubMed=11734900; DOI=10.1007/s00239-001-0019-8;
RA   Janke A., Magnell O., Wieczorek G., Westerman M., Arnason U.;
RT   "Phylogenetic analysis of 18S rRNA and the mitochondrial genomes of the
RT   wombat, Vombatus ursinus, and the spiny anteater, Tachyglossus aculeatus:
RT   increased support for the Marsupionta hypothesis.";
RL   J. Mol. Evol. 54:71-80(2002).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; AJ303116; CAC88019.1; -; Genomic_DNA.
DR   RefSeq; NP_542239.1; NC_003321.1.
DR   AlphaFoldDB; Q8W9G4; -.
DR   SMR; Q8W9G4; -.
DR   GeneID; 804509; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..459
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117992"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        435..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   459 AA;  51424 MW;  A6D7FF8B0BD1F08B CRC64;
     MLKILLPTVM LLPLISFSKK EWMWINSSAY SILISSLSLL TLNQHMDLGL NFNLNFFTDP
     LSSPLLVLSC WLLPLMILAS QFHLMNESTT HKRMYLILLV SLQVALLMAF SAVEFMMYYI
     LFETTLIPTL IIIARWGNQT ERLNAGLYFL FYTLLGSLPL LVALIFTQAQ MGSLHILLLT
     LTPNPLLNSW SNDILWLACM MAFLVKMPLY GFHLWLPKAH VEAPIAGSMV LAAILLKLGG
     YGILRIIIIL EPISKLMAYP FIILATWGMI MTSSICLRQT DLKSLIAYSS VSHMGLVVAA
     SLIQTPWGFM GATAMMIAHG LTSTMLFRLA NTNYERAHSR TMVLIRGLQM VLPLMSSWWL
     LASLANLGLP PTINLISELM IIVSAFSWSN LTLILLGLNT VITAIYSLYM LTSVQRGKMT
     THSLSINPTF TREHMIMALH LLPLILLTLN PKLILGVAY