ID   NU4M_TETNG              Reviewed;         460 AA.
AC   Q4JQH8;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon
OS   nigroviridis).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Tetraodon.
OX   NCBI_TaxID=99883;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17076253; DOI=10.1080/10425170600700378;
RA   Yue G.H., Lo L.C., Zhu Z.Y., Lin G., Feng F.;
RT   "The complete nucleotide sequence of the mitochondrial genome of Tetraodon
RT   nigroviridis.";
RL   DNA Seq. 17:115-121(2006).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; DQ019313; AAY26167.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q4JQH8; -.
DR   SMR; Q4JQH8; -.
DR   STRING; 99883.ENSTNIP00000002268; -.
DR   Ensembl; ENSTNIT00000003518; ENSTNIP00000002268; ENSTNIG00000001739.
DR   GeneTree; ENSGT00730000111316; -.
DR   HOGENOM; CLU_007100_4_0_1; -.
DR   InParanoid; Q4JQH8; -.
DR   OMA; ITRWGNQ; -.
DR   TreeFam; TF343520; -.
DR   Proteomes; UP000007303; Mitochondrion.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW   Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..460
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117994"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..331
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        437..457
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   460 AA;  51289 MW;  D786AFDE5C5CADDD CRC64;
     MLKILIPTVM LIPTAWLAPT KWLWPTTLLH SLLIALASLS WLKNASETGW SSLNPYMATD
     PLSTPLLILS CWLLPLMILA SQPNHTAHEP INRQRMYISL LTSLQFFLIL AFSATEMIMF
     YVMFEVTLIP TLILITRWGN QTERLNAGTY FLFYTLAGSL PLLVALLLLQ NSNGSLSLLT
     LLHSTPPQLS TYAAKIWWTG CILAFLVKMP LYGVHLWLPK AHVEAPIAGS MILAAVLLKL
     GGYGMMRILM TLEPLTKELS YPFIILALWG VIMTGSICMR QTDLKSLIAY SSVSHMGLVV
     GGILIQTPWG FTGALILMIA HGLTSSALFC LANTNYERTH SRTMLLARGL QMALPLMTAW
     WFIASLANLA LPPLPNLMGE LMIVTSLFNW SWWTIALTGL GMLITAGYSL YMFLMTQRGP
     LPSHIMALEP SHTREHLLIA LHLLPLLLLI LKPELIWGWS