NU4M_WHEAT
ID NU4M_WHEAT Reviewed; 495 AA.
AC P27572;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=ND4; Synonyms=NAD4;
OS Triticum aestivum (Wheat).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND RNA EDITING.
RC STRAIN=cv. Capitole; TISSUE=Etiolated seedling;
RX PubMed=1712098; DOI=10.1093/nar/19.12.3275;
RA Lamattina L., Grienenberger J.-M.;
RT "RNA editing of the transcript coding for subunit 4 of NADH dehydrogenase
RT in wheat mitochondria: uneven distribution of the editing sites among the
RT four exons.";
RL Nucleic Acids Res. 19:3275-3282(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 295-346, AND RNA EDITING.
RX PubMed=2687023; DOI=10.1016/0014-5793(89)81620-5;
RA Lamattina L., Weil J.H., Grienenberger J.M.;
RT "RNA editing at a splicing site of NADH dehydrogenase subunit IV gene
RT transcript in wheat mitochondria.";
RL FEBS Lett. 258:79-83(1989).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- RNA EDITING: Modified_positions=15 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 25 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 26 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 36 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 52 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 53 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 55 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 56 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 66 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 106 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 121 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 126 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 134 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 139 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 145 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 146 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 150 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 326 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 458 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 469 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 473 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023}, 478 {ECO:0000269|PubMed:1712098,
CC ECO:0000269|PubMed:2687023};
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; X57163; CAA40452.1; ALT_SEQ; mRNA.
DR EMBL; X57164; CAA40453.1; ALT_SEQ; Genomic_DNA.
DR PIR; S16447; S16447.
DR AlphaFoldDB; P27572; -.
DR SMR; P27572; -.
DR STRING; 4565.EPlTAEP00000010093; -.
DR eggNOG; KOG4845; Eukaryota.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IEA:UniProt.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 2: Evidence at transcript level;
KW Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW RNA editing; Translocase; Transmembrane; Transmembrane helix; Transport;
KW Ubiquinone.
FT CHAIN 1..495
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000118003"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 495 AA; 55898 MW; DCFF0B867527D628 CRC64;
MLEHFCECYF DLSGLILCPV LGSIILLFIP NSSIRLIRLI GLCVSLITFL YSLVLWIQFD
PSTAKFQFVE SLRWLPYENI HLYMGIDGLS LFFVILTTFL IPICILVGWS GMRSFGKEYI
IAFLICEFLM IAVFCMLDLL LFYVFFESVL IPMFIIIGVW GSRQRKIKAA YQFFLYTLLG
SVFMLLAILL ILLQTGTTDL QILLTTEFSE RRQILLWIAF FASFAVKVPM VPVHIWLPEA
HVEAPTAGSV ILAGILLKLG TYGFLRFSIP MFPEATLCFT PFIYTLSAIA IIYTSLTTLR
QIDLKKIIAY SSVAHMNLVT IGMFSLNIQG IGGSILLMLS HGLVSSALFL CVGVLYDRHK
TRLVRYYGGL VSTMPNFSTI FFFFTLANMS LPGTSSFIGE FLILVGAFQR NSLVATLRAL
GMILGAAYSL WLYNRVVSGN LKPDFLYKFS DLNGREVFIF LPFLVGVVWM GVYPKVFLDC
MHTSVSNLVQ HGKFH
