ID   NU4M_WICCA              Reviewed;         511 AA.
AC   P48917;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2002, sequence version 2.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Wickerhamomyces canadensis (Yeast) (Pichia canadensis).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX   NCBI_TaxID=1156965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=21;
RX   PubMed=8536312; DOI=10.1007/bf00311880;
RA   Sekito T., Okamoto K., Kitano H., Yoshida K.;
RT   "The complete mitochondrial DNA sequence of Hansenula wingei reveals new
RT   characteristics of yeast mitochondria.";
RL   Curr. Genet. 28:39-53(1995).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; D31785; BAA06575.2; -; Genomic_DNA.
DR   PIR; S58752; S58752.
DR   RefSeq; NP_038220.1; NC_001762.1.
DR   AlphaFoldDB; P48917; -.
DR   SMR; P48917; -.
DR   PRIDE; P48917; -.
DR   GeneID; 800541; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..511
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117939"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..309
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        318..338
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        388..408
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        422..442
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        476..496
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   511 AA;  58192 MW;  C87079CB5A5848E3 CRC64;
     MNQILIQGVI IIISLLSIII INQKDIILIN IKNKYIKQIS LLLIIYFIYI GIYINYLFNN
     NILGFQLINN YININWGIDG ISLWLINLTL ILLPISLISN WNISKNEDKS KVLTYVLLIL
     IIGIIIILNF ICLDLITFYI LFEATLLPLF ILIGKLGSLP YNGNNKIIIK GINNNNITPR
     EKAAYYIFIY TLFSSLFMLL SIGIYIYMIN NIDYNNIYNI ILSIDLQSII FIGLIIGILV
     KTPVFPVHTW LPLVHAESPI SGSIILAGII IKLAIYAIIR LILINLSDVI IIYNPLIYTI
     GILTIFYIGL ITLKQIDIKV IIAYSSIIHM AIAIMSIFSN NILGIEGSLL ISIAHGFASP
     ALFLLVGGIL YDRYHSRLIY YYQSLATYMP IFSIYLLIAG LFNMGIPLSL NFIGEFLSLN
     GIFIYNSLFA ILSTFSLFIT TIYQMKLTTK LLYGYKSIYI NIWSIWGNDV NKRELLLLNI
     LFFFIILFGI YPNLIIKSLN LSISSLLYIP F