ID   NU4M_XENLA              Reviewed;         461 AA.
AC   P03912;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=mt-nd4; Synonyms=mtnd4, nadh4, nd4;
OS   Xenopus laevis (African clawed frog).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=4019494; DOI=10.1016/s0021-9258(17)39303-1;
RA   Roe B.A., Ma D.-P., Wilson R.K., Wong J.F.-H.;
RT   "The complete nucleotide sequence of the Xenopus laevis mitochondrial
RT   genome.";
RL   J. Biol. Chem. 260:9759-9774(1985).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; M10217; AAA66467.1; -; Genomic_DNA.
DR   PIR; A00441; QXXL4M.
DR   RefSeq; NP_008143.1; NC_001573.1.
DR   AlphaFoldDB; P03912; -.
DR   SMR; P03912; -.
DR   MaxQB; P03912; -.
DR   GeneID; 2642077; -.
DR   KEGG; xla:2642077; -.
DR   CTD; 4538; -.
DR   Xenbase; XB-GENE-6251968; nd4.L.
DR   OrthoDB; 996580at2759; -.
DR   Proteomes; UP000186698; Mitochondrion MT.
DR   Bgee; 2642077; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW   Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..461
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000118004"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   461 AA;  52043 MW;  E75BAA47685DAC9B CRC64;
     MLKILLPTLM LIPSTWLTNK KWLWPSLTSQ SLIISLLSLM WFFNQSETTH FSNYLMTIDQ
     ISTPLLILTC WLLPLMLIAS QNHLSNEPIS RQRTFITMLV FLQLSLIMAF SATELILFYI
     MFEITLIPTL IIITRWGNQA ERLNAGTYFL FYTLAGSLPL LVALLSLYSS TGTLSLNLLQ
     LLPNHIPMTW ANYSWWLACL LAFMVKMPLY GTHLWLPKAH VEAPIAGSMV LAAILLKLGG
     YGIIRISITL SPSMKELAYP FLILSLWGII MTSSICLRQT DLKSMIAYSS VSHMGLVISA
     GNNQTPMKAL TGAMILNTSD GLTHSALCCL AKYQSYERTH SRALLLSRGL ETILPLMGTW
     WLISNLANMA LPPSPNWMGE ITIMTALFNW SSWTIILTDL GTLLTASYSL YMFLMTQRGM
     TPEHLNAINP THTREHTLMT MHLIPIIPLM MKPELIWGLF F