NU4M_YARLI
ID NU4M_YARLI Reviewed; 486 AA.
AC Q9B6D6;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=ND4;
OS Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Dipodascaceae; Yarrowia.
OX NCBI_TaxID=284591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 20460 / W29 / CBS 7504 / IFP29;
RX DOI=10.1002/cfg.72;
RA Kerscher S., Durstewitz G., Casaregola S., Gaillardin C., Brandt U.;
RT "The complete mitochondrial genome of Yarrowia lipolytica.";
RL Comp. Funct. Genomics 2:80-90(2001).
RN [2]
RP SUBUNIT.
RX PubMed=15282186; DOI=10.1016/j.bbabio.2004.04.019;
RA Abdrakhmanova A., Zickermann V., Bostina M., Radermacher M., Schagger H.,
RA Kerscher S., Brandt U.;
RT "Subunit composition of mitochondrial complex I from the yeast Yarrowia
RT lipolytica.";
RL Biochim. Biophys. Acta 1658:148-156(2004).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBUNIT: Complex I is composed of 37 different subunits.
CC {ECO:0000269|PubMed:15282186}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AJ307410; CAC28103.2; -; Genomic_DNA.
DR PIR; S51503; S51503.
DR RefSeq; NP_075436.2; NC_002659.1.
DR PDB; 6GCS; EM; 4.32 A; 4=1-486.
DR PDB; 6H8K; X-ray; 3.79 A; 4=136-471.
DR PDB; 6RFQ; EM; 3.30 A; 4=1-486.
DR PDB; 6RFR; EM; 3.20 A; 4=1-486.
DR PDB; 6RFS; EM; 4.04 A; 4=1-486.
DR PDB; 6Y79; EM; 3.20 A; 4=1-486.
DR PDB; 6YJ4; EM; 2.70 A; M=1-486.
DR PDBsum; 6GCS; -.
DR PDBsum; 6H8K; -.
DR PDBsum; 6RFQ; -.
DR PDBsum; 6RFR; -.
DR PDBsum; 6RFS; -.
DR PDBsum; 6Y79; -.
DR PDBsum; 6YJ4; -.
DR AlphaFoldDB; Q9B6D6; -.
DR SMR; Q9B6D6; -.
DR DIP; DIP-61439N; -.
DR IntAct; Q9B6D6; 2.
DR STRING; 284591.Q9B6D6; -.
DR GeneID; 802604; -.
DR KEGG; yli:YalifMp16; -.
DR InParanoid; Q9B6D6; -.
DR Proteomes; UP000001300; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion; NAD;
KW Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..486
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000118005"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 3..20
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 25..44
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 54..60
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 65..67
FT /evidence="ECO:0007829|PDB:6RFR"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 83..101
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 111..129
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 134..141
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 144..155
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:6RFQ"
FT HELIX 160..189
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 194..196
FT /evidence="ECO:0007829|PDB:6YJ4"
FT TURN 197..199
FT /evidence="ECO:0007829|PDB:6RFR"
FT HELIX 204..221
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 225..227
FT /evidence="ECO:0007829|PDB:6RFR"
FT HELIX 230..237
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 240..261
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 263..266
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 267..292
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 298..317
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 322..347
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 350..354
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 359..361
FT /evidence="ECO:0007829|PDB:6YJ4"
FT TURN 365..368
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 370..383
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 390..405
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 407..412
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 413..415
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 416..432
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 433..435
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 438..440
FT /evidence="ECO:0007829|PDB:6RFR"
FT HELIX 448..466
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 468..474
FT /evidence="ECO:0007829|PDB:6YJ4"
FT TURN 475..477
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 478..481
FT /evidence="ECO:0007829|PDB:6YJ4"
SQ SEQUENCE 486 AA; 54481 MW; 40F8C1FB1A2A02D5 CRC64;
MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF
NSLNPFGLSN SDISNGLLFG IDGLSLTFIL LTVLLIPLTL LGNWYNINFN SNLYYTLVLA
IGLVILLNFW ALDYISFYIL FEATLPLLFI LIHIYGSSDS ERASFYVLMF TLSGSLFMLL
SIVVISIVLN TTNFINHNLF VLSLDLQTII WLGLFIAIMV KTPLFPIHVW LPVVHSESPL
AGSMILAGLI LKLALYAILR LLLPLLCEAQ ILYTPMIYII SLLTIILTSL ATLRQIDLKV
IIAYSSISHM GIAILGVCSN TSLGIYGSIV LGVAHGFVSP ALFLIVGGIL YDRYHIRIVN
YYKGLTTYMP QLATYIIILS FANIGTPLTG NFTGEFLSLQ GGFIRNPIIG GISCISVLLA
AIYQLKLTNK LTGGISSIYM HRTNDVTIRE KFIMNILIIS TLIIGICPQI MYNLLYWTVN
NYIYII
