NU5C_ARATH
ID NU5C_ARATH Reviewed; 746 AA.
AC P56752; Q9MS93;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic;
DE EC=7.1.1.-;
DE AltName: Full=NAD(P)H dehydrogenase subunit 5;
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit 5;
GN Name=ndhF; OrderedLocusNames=AtCg01010;
OS Arabidopsis thaliana (Mouse-ear cress).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10574454; DOI=10.1093/dnares/6.5.283;
RA Sato S., Nakamura Y., Kaneko T., Asamizu E., Tabata S.;
RT "Complete structure of the chloroplast genome of Arabidopsis thaliana.";
RL DNA Res. 6:283-290(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-426.
RA Graham S.W., Reeves P.A., Burns A., Olmstead R.G.;
RT "Long branches in the seed plants and the root of the angiosperms.";
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which
CC are encoded in the nucleus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; AP000423; BAA84434.1; -; Genomic_DNA.
DR EMBL; AF238049; AAF90035.1; -; Genomic_DNA.
DR RefSeq; NP_051106.1; NC_000932.1.
DR PDB; 7WFF; EM; 3.59 A; F=1-746.
DR PDB; 7WG5; EM; 3.89 A; F=1-746.
DR PDBsum; 7WFF; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; P56752; -.
DR SMR; P56752; -.
DR STRING; 3702.ATCG01010.1; -.
DR TCDB; 3.D.1.8.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P56752; -.
DR PRIDE; P56752; -.
DR ProteomicsDB; 249042; -.
DR EnsemblPlants; ATCG01010.1; ATCG01010.1; ATCG01010.
DR GeneID; 844705; -.
DR Gramene; ATCG01010.1; ATCG01010.1; ATCG01010.
DR KEGG; ath:ArthCp071; -.
DR Araport; ATCG01010; -.
DR TAIR; locus:504954724; ATCG01010.
DR eggNOG; KOG4668; Eukaryota.
DR HOGENOM; CLU_007100_6_0_1; -.
DR InParanoid; P56752; -.
DR OMA; QIFITVE; -.
DR OrthoDB; 526738at2759; -.
DR BioCyc; ARA:ATCG01010-MON; -.
DR PRO; PR:P56752; -.
DR Proteomes; UP000006548; Chloroplast.
DR ExpressionAtlas; P56752; baseline and differential.
DR Genevisible; P56752; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR002128; NADH_UbQ_OxRdtase_chlpt_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF01010; Proton_antipo_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone;
KW Quinone; Reference proteome; Thylakoid; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..746
FT /note="NAD(P)H-quinone oxidoreductase subunit 5,
FT chloroplastic"
FT /id="PRO_0000118169"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 547..567
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 608..628
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 723..743
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 746 AA; 85239 MW; 1391A7875E9E7A29 CRC64;
MEHTYQYSWI IPFIPLPVPI LLGVGLLLFP TATKNLRRMW TFLSIFLLSI VMIFSIYLSI
QQIFLSCIHQ NVWSWTINNE FSFEFGYFID PLTSIMSILI TTVGILVLIY SDNYMSHDQG
YLRFFAYMGF FNTSMLGLVT SSNLIQVYFF WELVGMCSYL LIGFWFTRPI AANACQKAFV
TNRVGDFGLL LGILGLYWIT GSFEFQDLFE IFNNLILNNR VNLLFLTLCA FLLFVGPIAK
SAQFPLHVWL PDAMEGPTPI SALIHAATMV AAGIFLVARL LPLFIVIPSI MYIISLIGII
TVLLGATLAL AQKDIKRGLA YSTMSQLGYM MLALGMGSYR SALFHLITHA YSKALLFLGS
GSIIHSMEAI VGYSPDKSQN MILMGGLTKH VPITKTAFLI GTLSLCGIPP LACFWSKDEI
LNDSLLFSPI FAIIACSTAG LTAFYMFRIY LLTFEGHLNT YFLNYSGKKS GSFYSLSLWG
KEEEKKLNKN FGLVPLLTMN NTKRASFFCN KTYKISNNVR NQIFITVENF GLNTRTFYYP
HESDNTILFP MLILVLFTLF IGAIGIPFNQ EGIDFDILSK FFTPSINLLH KNSQNFVDWY
EFLRNATFSV SIAFFGIFIA YCLYKPFYSS LLNLTLLNSF QKWNSKRIHW EKLINFVYNW
SYNRGYIDSF FKTSLIESIR RLAKQTTFFD KRIIDGITNG VGITSFFVGE VTKYIGGSRI
SSYLFLYLSY VLIFLMILFF FYFEKF
