NU5C_ATRBE
ID NU5C_ATRBE Reviewed; 740 AA.
AC Q8S8V0; Q31773;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic;
DE EC=7.1.1.-;
DE AltName: Full=NAD(P)H dehydrogenase subunit 5;
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit 5;
GN Name=ndhF;
OS Atropa belladonna (Belladonna) (Deadly nightshade).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Hyoscyameae; Atropa.
OX NCBI_TaxID=33113;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Ab5p(kan);
RX PubMed=12200487; DOI=10.1093/oxfordjournals.molbev.a004222;
RA Schmitz-Linneweber C., Regel R., Du T.G., Hupfer H., Herrmann R.G.,
RA Maier R.M.;
RT "The plastid chromosome of Atropa belladonna and its comparison with that
RT of Nicotiana tabacum: the role of RNA editing in generating divergence in
RT the process of plant speciation.";
RL Mol. Biol. Evol. 19:1602-1612(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-703.
RA Olmstead R.G., Sweere J.A.;
RT "Combining data in phylogenetic systematics: an empirical approach using
RT three molecular data sets in the Solanaceae.";
RL Syst. Biol. 43:467-481(1994).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which
CC are encoded in the nucleus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; AJ316582; CAC88093.1; -; Genomic_DNA.
DR EMBL; U08915; AAA18597.2; -; Genomic_DNA.
DR RefSeq; NP_783279.1; NC_004561.1.
DR AlphaFoldDB; Q8S8V0; -.
DR SMR; Q8S8V0; -.
DR GeneID; 806500; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR002128; NADH_UbQ_OxRdtase_chlpt_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF01010; Proton_antipo_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 3: Inferred from homology;
KW Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone; Quinone;
KW Thylakoid; Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..740
FT /note="NAD(P)H-quinone oxidoreductase subunit 5,
FT chloroplastic"
FT /id="PRO_0000118172"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 543..563
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 718..738
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 62
FT /note="Q -> Z (in Ref. 2; AAA18597)"
FT /evidence="ECO:0000305"
FT CONFLICT 568
FT /note="V -> W (in Ref. 2; AAA18597)"
FT /evidence="ECO:0000305"
FT CONFLICT 670
FT /note="F -> L (in Ref. 2; AAA18597)"
FT /evidence="ECO:0000305"
FT CONFLICT 674
FT /note="I -> L (in Ref. 2; AAA18597)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 740 AA; 83956 MW; A72CAE65EA7B70C2 CRC64;
MEQTYEYAWI IPFIPLPVPM LIGAGLILFP TATKSFRRMW AFQSVLLLSI VMIFSIYLSI
QQINSSSFYQ YVWSWIINND FSLDFGYLID PLTSIMSILI TTVGIMVLIY SDNYMAHDQG
YLRFFAYMSF FSTSMLGLVT SSNLIQIYIF WELVGLCSYL LIGFWFTRPV AANACQKAFV
TNRVGDFGLL LGILGFYWIT GSFEFRDLFE IFNNLSSNNE VNFLFVTLCA VLLFAGAVAK
SAQFPLHVWL PDAMEGPTPI SALIHAATMV AAGIFLVARL LPLFRVIPYI MYLISVIGII
TVLLGATLAL AQKDIKRGLA YSTMSQLGYM MLALGMGSYR SALFHLITHA YSKALLFLGS
GSIIHSMETI VGYSPAKSQN MGLMGGLRKH VPITQITFLL GTLSLCGIPP LACFWSKDEI
LNDSWLYSPI FAIIAWATAG LTAFYMFRIY LLTFEGHLNV HFQNYGGKQK TPFYSISLWG
KKGVKKNSYL LTMNNNESTY FFSKTKYPID KNGRKMTRPF MTIAHFEHKT VYSYPYESDN
TMLFPIFVLG LFTLFVGSIG IPFNQEGVNL DILSKWLAPS INLLHQKSNN SMDWNEFLKD
AVLSVSIAYF GIFLASFLYK PIYSSLKNFE LINSFVKKGP KRILWDKIIN GIYDWSYNRA
YIDAFYTRFF VGGIRGLAEF IHFFDRRVID GMTNGVGVIS FIVGEGIKYI GGGRISSYLF
LYLAYVSVFL LVYYLFFLTF
