NU5C_VICFA
ID NU5C_VICFA Reviewed; 746 AA.
AC P15958;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic;
DE EC=7.1.1.-;
DE AltName: Full=NAD(P)H dehydrogenase subunit 5;
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit 5;
GN Name=ndhF;
OS Vicia faba (Broad bean) (Faba vulgaris).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Vicia.
OX NCBI_TaxID=3906;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2320425; DOI=10.1093/nar/18.5.1297;
RA Herdenberger F., Pillay D.T.N., Steinmetz A.;
RT "Sequence of the trnH gene and the inverted repeat structure deletion site
RT of the broad bean chloroplast genome.";
RL Nucleic Acids Res. 18:1297-1297(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3242868; DOI=10.1007/bf00434087;
RA Herdenberger F., Weil J.H., Steinmetz A.;
RT "Organization and nucleotide sequence of the broad bean chloroplast genes
RT trnL-UAG, ndhF and two unidentified open reading frames.";
RL Curr. Genet. 14:609-615(1988).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which
CC are encoded in the nucleus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; X51471; CAA35834.1; -; Genomic_DNA.
DR EMBL; X14804; CAA32909.1; -; Genomic_DNA.
DR EMBL; M36832; AAA84714.1; -; Genomic_DNA.
DR PIR; S08494; S08494.
DR AlphaFoldDB; P15958; -.
DR SMR; P15958; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR002128; NADH_UbQ_OxRdtase_chlpt_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF01010; Proton_antipo_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 3: Inferred from homology;
KW Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone; Quinone;
KW Thylakoid; Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..746
FT /note="NAD(P)H-quinone oxidoreductase subunit 5,
FT chloroplastic"
FT /id="PRO_0000118207"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..626
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 726..746
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 746 AA; 84768 MW; 252471990B47CF9C CRC64;
MEYTHQSSWI IPFIPLPVPI LIGVGLLLFP TATKNLRRMW AFPSIFLLTI VMIFSIDLSI
QQIENSSIYQ YVWSWTINND LSLEFGYLID SLTSIMSILI TTVGILVLIY SDSYMSHDQG
YLRFFTYMSF FNTSMLGLVT SSNLIQVYIF WELVGMCSYL LIGFWFTRPI AANACQKAFV
TNRVGDFGLL LGILGFYWIT GSLEFRDLFQ IFKNLIYKNE VNILFVTLCA LLLFCGSVAK
SAQFPLHVWL PDAMEGPTPI SALIHAATMV AAGIFLVARL LPLFIVIPSI MSGIALIGII
TVVLGATLAI AQKDIKKNLA YSTMSQLGYM MLALGMGSYR AALFHLITHA YSKALLFLGS
GSIIHSMEAI VGYSPDKSQN MVLMGGLTKH APITKMSFLI GTLSLCGIPP FACFWSKDEI
LNDSWLYSPI FAIIACSTAG LTAFYMFRIY LLVFEGYLNV HFQNFNGKKN SSFYSISLWG
KEEKKKLKKK IHLLGFLTMN NNERTSFFRE RTYSHRINRN VKSIRRLFLD STHFGTKNLP
FFYPHESDNT MLFSMLVLVL FTFFVGSIGI SFSQEGIDLD ILSKLLIPSI DLLHQNSKNS
VDWYEFFINA TFSVSIAFFG LFIASFFYKP VFSSLQNLNL FNLFQKNVPK KIISDKIINI
LYDWSYNRGY IDAFFEVSLI ASVRKLAKFN YFFDRQLIDG IPNGVGISNF FIGEAIKYVG
GGRISSYIFF FVLIFLLICY YIYLFP
