NU5M_ARATH
ID NU5M_ARATH Reviewed; 669 AA.
AC P29388;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=ND5; Synonyms=NAD5, NAD5C;
GN OrderedLocusNames=AtMg00060/AtMg00513/AtMg00665;
OS Arabidopsis thaliana (Mouse-ear cress).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND RNA EDITING.
RC STRAIN=cv. Columbia;
RX PubMed=1915303; DOI=10.1002/j.1460-2075.1991.tb04912.x;
RA Knoop V., Schuster W., Wissinger B., Brennicke A.;
RT "Trans splicing integrates an exon of 22 nucleotides into the nad5 mRNA in
RT higher plant mitochondria.";
RL EMBO J. 10:3483-3493(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=8988169; DOI=10.1038/ng0197-57;
RA Unseld M., Marienfeld J.R., Brandt P., Brennicke A.;
RT "The mitochondrial genome of Arabidopsis thaliana contains 57 genes in
RT 366,924 nucleotides.";
RL Nat. Genet. 15:57-61(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND RNA EDITING.
RX PubMed=10611383; DOI=10.1073/pnas.96.26.15324;
RA Giege P., Brennicke A.;
RT "RNA editing in Arabidopsis mitochondria effects 441 C to U changes in
RT ORFs.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBUNIT: Complex I is composed of at least 49 different subunits.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- RNA EDITING: Modified_positions=52 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 81 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 120 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 125 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 133 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 165 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 183 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 185 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 200 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 203 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 210 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 226 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 238 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 242 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 255 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 279 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 292 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 497 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 517 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 527 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 632 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 639 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 640 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303}, 653 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:1915303};
CC -!- MISCELLANEOUS: Exons a and b (AtMg00513) and d and e (AtMg00060) are
CC cis-spliced, while trans-splicing reactions are required to link exons
CC b and c (AtMg00665) and c and d.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; X60045; CAA42648.1; ALT_SEQ; mRNA.
DR EMBL; X60047; CAA42648.1; JOINED; mRNA.
DR EMBL; X60048; CAA42648.1; JOINED; mRNA.
DR EMBL; Y08501; CAA69752.3; ALT_SEQ; Genomic_DNA.
DR PIR; S20234; DNMUU5.
DR RefSeq; NP_085478.1; NC_001284.2.
DR PDB; 7A23; EM; 3.70 A; a=1-669.
DR PDB; 7AR7; EM; 3.72 A; L=1-615.
DR PDBsum; 7A23; -.
DR PDBsum; 7AR7; -.
DR AlphaFoldDB; P29388; -.
DR SMR; P29388; -.
DR IntAct; P29388; 2.
DR STRING; 3702.ATMG00060.1; -.
DR PaxDb; P29388; -.
DR PeptideAtlas; P29388; -.
DR PRIDE; P29388; -.
DR ProteomicsDB; 250554; -.
DR Araport; ATMG00060; -.
DR Araport; ATMG00513; -.
DR Araport; ATMG00665; -.
DR eggNOG; KOG4668; Eukaryota.
DR HOGENOM; CLU_1500061_0_0_1; -.
DR InParanoid; P29388; -.
DR OrthoDB; 526738at2759; -.
DR BioCyc; ARA:ATMG00513-MON; -.
DR BioCyc; MetaCyc:ATMG00513-MON; -.
DR PRO; PR:P29388; -.
DR Proteomes; UP000006548; Mitochondrion (cv. C24).
DR ExpressionAtlas; P29388; baseline and differential.
DR Genevisible; P29388; AT.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0009536; C:plastid; IEA:UniProt.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW RNA editing; Translocase; Transmembrane; Transmembrane helix; Transport;
KW Ubiquinone.
FT CHAIN 1..669
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118059"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 619..639
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 669 AA; 74316 MW; DB241A65EF0BBBBF CRC64;
MYLLIVFLPL LGSSVAGFFG RFLGSEGSAI MTTTCVSFSS ILSLIAFYEV ALGASACYLR
IAPWISSEMF DASWGFLFDS LTVVMLIVVT SISSLVHLYS ISYMSEDPHS PRFMCYLSIF
TFFMLMLVTG DNFLQLFLGW EGVGLASYLL IHFWFTRLQA DKAAIKAMLV NRVGDFGLAL
GILGCFTLFQ TVDFSTIFAC ASVPRNSWIF CNMRLNAISL ICILLFIGAV GKSAQIGLHT
WLPDAMEGPT PVSALIHAAT MVTAGVFMIA RCSPLFEYSP TALIVITSAG AMTSFLAATT
GILQNDLKRV IAYSTCSQLG YMIFACGISN YSVSVFHLMN HAFFKALLFL SAGSVIHAMS
DEQDMRKMGG LASSFPLTYA MMLIGSLSLI GFPFLTGFYS KDVILELAYT KYTISGNFAF
WLGSVSVLFT SYYSFRLLFL TFLVPTNSFG RDISRCHDAP IPMAIPLILL ALGSLFVGYL
AKDMMIGLGT NFWANSLLVL PKNEILAESE FAAPTIIKLI PILFSTLGAF VAYNVNPVAD
QFQRAFQTST FCNRLYSFFN KRWFFDQVLN DFLVRSFLRF GYEVSFEALD KGAIEILGPY
GISYTFRRLA ERISQLQSGF VYHYAFAMLL GLTLFVTFFC MWDSLSSWVD NRLSFILIVS
SFYTKSSQE
