NU5M_CHLRE
ID NU5M_CHLRE Reviewed; 546 AA.
AC P08739; Q34228;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=ND5; Synonyms=NAD5;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RX PubMed=3020517; DOI=10.1093/nar/14.18.7506;
RA Boer P.H., Gray M.W.;
RT "Nucleotide sequence of a protein coding region in Chlamydomonas
RT reinhardtii mitochondrial DNA.";
RL Nucleic Acids Res. 14:7506-7507(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RA Gray M.W.;
RL Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RA Vahrenholz C., Pratje E., Michaelis G., Dujon B.;
RT "Mitochondrial DNA of Chlamydomonas reinhardtii: sequence and arrangement
RT of URF5 and the gene for cytochrome oxidase subunit I.";
RL Mol. Gen. Genet. 201:213-224(1985).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RX PubMed=1831072; DOI=10.1007/bf00355060;
RA Boer P.H., Gray M.W.;
RT "Short dispersed repeats localized in spacer regions of Chlamydomonas
RT reinhardtii mitochondrial DNA.";
RL Curr. Genet. 19:309-312(1991).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RX PubMed=3007117; DOI=10.1002/j.1460-2075.1986.tb04172.x;
RA Boer P.H., Gray M.W.;
RT "The URF 5 gene of Chlamydomonas reinhardtii mitochondria: DNA sequence and
RT mode of transcription.";
RL EMBO J. 5:21-28(1986).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA38641.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X54860; CAA38641.1; ALT_INIT; Genomic_DNA.
DR EMBL; U03843; AAB93442.1; -; Genomic_DNA.
DR EMBL; X66484; CAA47113.1; -; Genomic_DNA.
DR EMBL; X03464; CAA27181.1; -; Genomic_DNA.
DR PIR; B24707; B24707.
DR RefSeq; NP_042566.1; NC_001638.1.
DR AlphaFoldDB; P08739; -.
DR SMR; P08739; -.
DR STRING; 3055.AAB93442; -.
DR TCDB; 3.D.1.6.4; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR GeneID; 801492; -.
DR KEGG; cre:ChrepMp03; -.
DR eggNOG; KOG4668; Eukaryota.
DR HOGENOM; CLU_007100_6_0_1; -.
DR OrthoDB; 526738at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..546
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118080"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 1
FT /note="M -> MSSSLVQINELANREISLLFPM (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="G -> A (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="I -> S (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 546 AA; 59048 MW; B7AA04BBB53B17DD CRC64;
MFLLSVFFPL LGGLVNTSPI ARFLGHRGSS IIAIGCMVVA FISSVVIYYE VVFMGCAVSV
DVFGTWFSVG TFHAGWTFNF DLLTANMLFT VTGVSMAVHM YACDYMRQDP HLNLFLGYLS
YFTGFMCVLV AADNLLVMLV GWEGIGVCSY LLIGYWSHRL SAVKSAQKAI LVNRVSDGLL
MWGVLWVWYH LGSLEYDLLN VYSASGFVGL SILIGAMGKS AQILFHVWLA DAMEGPTPVS
ALIHAATLVT AGVYLLVRLH IHDEMFVIIV GSLTAFMAGV FGATQSDLKR VIAYSTCSQL
GYMMVSLGLG ETGGEASMGH LMTHASFKAA LFLAAGMVIS GNGGNQHIAR YGGSAHSAMF
TMLTLMVASL SLIGWPELSG FYSKETILNL AAICADPIAD VAHTLLLLTA MLTSAYTTKL
FYQCFMVDFS GSSVTPVRNV LPILAMAILL LDIMLKVWVG TNLLSGMLFF LPWGVKTLPF
GLMVAGILTA TAAVGSERFT LIRFCGSRWG FDQLFARSPV NPIFDLGRIT WAIGDRGLLS
VGNLRA
