NU5M_DICDI
ID NU5M_DICDI Reviewed; 670 AA.
AC Q34313; Q9T447;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=nad5; ORFNames=DDB_G0294026;
OS Dictyostelium discoideum (Slime mold).
OG Mitochondrion.
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX4;
RA Sucgang R., Muzny D., Gibbs R., Kuspa A.;
RT "The Dictyostelium genome project: complete annotation of the mitochondrial
RT DNA.";
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX3;
RX PubMed=10821186; DOI=10.1007/pl00008685;
RA Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K.,
RA Morio T., Urushihara H., Yanagisawa K., Tanaka Y.;
RT "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene
RT content and genome organization.";
RL Mol. Gen. Genet. 263:514-519(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-232.
RC STRAIN=X22;
RX PubMed=7807547; DOI=10.1007/bf00160403;
RA Cole R.A., Williams K.L.;
RT "The Dictyostelium discoideum mitochondrial genome: a primordial system
RT using the universal code and encoding hydrophilic proteins atypical of
RT metazoan mitochondrial DNA.";
RL J. Mol. Evol. 39:579-588(1994).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; AF155828; AAD43330.1; -; Genomic_DNA.
DR EMBL; AB000109; BAA78087.1; -; Genomic_DNA.
DR EMBL; U02291; AAA77668.1; -; Genomic_DNA.
DR PIR; T43784; T43784.
DR RefSeq; NP_050105.1; NC_000895.1.
DR AlphaFoldDB; Q34313; -.
DR SMR; Q34313; -.
DR GeneID; 2193930; -.
DR KEGG; ddi:DidioMp38; -.
DR dictyBase; DDB_G0294026; nad5.
DR InParanoid; Q34313; -.
DR OMA; LIGFWQH; -.
DR PhylomeDB; Q34313; -.
DR PRO; PR:Q34313; -.
DR Proteomes; UP000002195; Mitochondrion.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..670
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118087"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 519..539
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 594..614
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 629..649
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 650..670
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 670 AA; 75460 MW; 63855B60D8A26AF8 CRC64;
MYIVNLILPL IGSIITGIFG HKLGNRISIK IAVGCMMLTA ISSLYIGYEI LLCNSVVHFK
LGTWMQVGSL NVEYGLLYDS LTSIMIIVIT CISSMVHLYS MDYMKEDPHK TRFFSYLSLF
TFFMMLLVTA DNFVQLFFGW EGVGIMSYLL INFWYTRLQA NKSALKAVIL NRFGDFGLFF
GILLVFLVFK SVDFSVIFTI APFITEYTIN LLGYEVNAIT LIGSFIVIGV VGKSAQLGLH
MWLPDAMEGP TPVSALLHAA TMVTAGVFLV LRTSPLLSYS ITILNILTII GALTTLFATT
IGIVQNDIKR VIAYSTCSQL GYMIFACGLL NYNASIYHLT THAFFKALLF LSAGSVIHGL
NDEQDMRKMG GLVNLMPLTY QCMLIGTLAL TGFPFLSGYY SKDIILETSY ATYYWEGTFA
AIIGYVAAFG TTFYSFRLLI LTFFNKPRMQ YKTIAGVHEA STNMVIPLVI LALCSIFIGY
VTKDFFVGLG TPVWNNSFFA YPYNNLILES EVLQRELKLL PLFAFIYGVI TPVLFYFNIK
EDRMINVKQN LMVKESYFFF VKKWYFDFLS RVLIVVPFFH LSYDVMNKNL DKGLWEKIGV
TGVATTLVTA FTALKLKNEI TLSTYISYIV QTIILIIVVG IFSFMTGFIY MELCIIIGIL
YICLPSIKID
