NU5M_DROME
ID NU5M_DROME Reviewed; 572 AA.
AC P18932; Q36735; Q9MGN9; Q9MJC7;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 4.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=mt:ND5; Synonyms=NADH5, ND5;
OS Drosophila melanogaster (Fruit fly).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bretagne;
RX PubMed=3130291; DOI=10.1093/genetics/118.4.649;
RA Garesse R.;
RT "Drosophila melanogaster mitochondrial DNA: gene organization and
RT evolutionary considerations.";
RL Genetics 118:649-663(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R, and Zimbabwe 53;
RX PubMed=10903372; DOI=10.1007/s002390010066;
RA Ballard J.W.O.;
RT "Comparative genomics of mitochondrial DNA in members of the Drosophila
RT melanogaster subgroup.";
RL J. Mol. Evol. 51:48-63(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Paris;
RX PubMed=11298822; DOI=10.1046/j.1365-2540.2001.00814.x;
RA Azou Y., Bregliano J.C.;
RT "I-R system of hybrid dysgenesis in Drosophila melanogaster: analysis of
RT the mitochondrial DNA in reactive strains exhibiting different potentials
RT for I factor transposition.";
RL Heredity 86:110-116(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8825764; DOI=10.1111/j.1365-2583.1995.tb00032.x;
RA Lewis D.L., Farr C.L., Kaguni L.S.;
RT "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide
RT sequence and evolutionary comparisons.";
RL Insect Mol. Biol. 4:263-278(1995).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RA Wan K., Celniker S.;
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-510.
RX PubMed=7851771; DOI=10.1093/genetics/138.3.741;
RA Rand D.M., Dorfsman M., Kann L.M.;
RT "Neutral and non-neutral evolution of Drosophila mitochondrial DNA.";
RL Genetics 138:741-756(1994).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB91058.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M37275; AAA69710.1; ALT_TERM; Genomic_DNA.
DR EMBL; AF200828; AAF77233.1; -; Genomic_DNA.
DR EMBL; AF200829; AAF77251.1; -; Genomic_DNA.
DR EMBL; AJ400907; CAB91058.1; ALT_INIT; Genomic_DNA.
DR EMBL; U37541; AAC47818.1; -; Genomic_DNA.
DR EMBL; KJ947872; AIC64011.1; -; Genomic_DNA.
DR EMBL; S76764; AAB33356.1; -; Genomic_DNA.
DR PIR; S01186; S01186.
DR RefSeq; YP_009047273.1; NC_024511.2.
DR AlphaFoldDB; P18932; -.
DR SMR; P18932; -.
DR BioGRID; 2595078; 1.
DR STRING; 7227.FBpp0100182; -.
DR PaxDb; P18932; -.
DR EnsemblMetazoa; FBtr0433501; FBpp0390633; FBgn0013684.
DR GeneID; 19893549; -.
DR KEGG; dme:Dmel_CG34083; -.
DR CTD; 4540; -.
DR FlyBase; FBgn0013684; mt:ND5.
DR VEuPathDB; VectorBase:FBgn0013684; -.
DR eggNOG; KOG4668; Eukaryota.
DR GeneTree; ENSGT00730000111303; -.
DR HOGENOM; CLU_007100_6_0_1; -.
DR InParanoid; P18932; -.
DR OrthoDB; 526738at2759; -.
DR PhylomeDB; P18932; -.
DR Reactome; R-DME-611105; Respiratory electron transport.
DR Reactome; R-DME-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 19893549; 0 hits in 1 CRISPR screen.
DR ChiTaRS; ND5; fly.
DR GenomeRNAi; 19893549; -.
DR PRO; PR:P18932; -.
DR Proteomes; UP000000803; Mitochondrion.
DR Bgee; FBgn0013684; Expressed in midgut and 14 other tissues.
DR ExpressionAtlas; P18932; baseline and differential.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:FlyBase.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..572
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118090"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 331
FT /note="I -> IGI (in Ref. 1; AAA69710/AAC47818)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 572 AA; 65145 MW; 8BFEEF4CE24B5575 CRC64;
MCSISFVNLI SMSLSCFLLS LYFLLNDMIY FIEWELVSLN SMSIVMTFLF DWMSLLFMSF
VLMISSLVIF YSKEYMMNDN HINRFIMLVL MFVLSMMLLI ISPNLISILL GWDGLGLVSY
CLVIYFQNIK SYNAGMLTAL SNRIGDVALL LSIAWMLNYG SWNYIFYLEI MQNEFEMLMI
GSLVMLAAMT KSAQIPFSSW LPAAMAAPTP VSALVHSSTL VTAGVYLLIR FNIILSTSWL
GQLMLLLSGL TMFMAGLGAN FEFDLKKIIA LSTLSQLGLM MSILSMGFLK LAMFHLLTHA
LFKALLFMCA GAIIHNMNNS QDIRLMGGLS IHMPLTSACF NVSNLALCGM PFLAGFYSKD
MILEIVSISN VNMFSFFLYY FSTGLTVSYS FRLVYYSMTG DLNCGSLNML NDESWIMLRG
MMGLLIMSII GGSMLNWLIF PFPYMICLPI YMKLLTLFVC IVGGLFGYLI SLSNLFFLNK
SLFMYNLSTF LGSMWFMPYI STYGMIFYPL NYGQLVVKSF DQGWSEYFGG QHLYQKLSMY
SKTLFLMHNN SLKIYLLLFV FWILILLILL FL
