NU5M_METSE
ID NU5M_METSE Reviewed; 600 AA.
AC Q35099;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=ND5;
OS Metridium senile (Brown sea anemone) (Frilled sea anemone).
OG Mitochondrion.
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Nynantheae; Metridiidae; Metridium.
OX NCBI_TaxID=6116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=White morph;
RX PubMed=8643626; DOI=10.1073/pnas.93.11.5619;
RA Beagley C.T., Okada N.A., Wolstenholme D.R.;
RT "Two mitochondrial group I introns in a metazoan, the sea anemone Metridium
RT senile: one intron contains genes for subunits 1 and 3 of NADH
RT dehydrogenase.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:5619-5623(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=White morph;
RA Beagley C.T., Okimoto R., Wolstenholme D.R.;
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U36784; AAC47102.1; -; Genomic_DNA.
DR EMBL; AF000023; AAC04635.1; -; Genomic_DNA.
DR PIR; T11889; T11889.
DR RefSeq; NP_009258.1; NC_000933.1.
DR AlphaFoldDB; Q35099; -.
DR SMR; Q35099; -.
DR GeneID; 808773; -.
DR CTD; 4540; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..600
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118113"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 520..540
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 600 AA; 66448 MW; 0B175EAE5E3A5799 CRC64;
MYILVLTVPL LGALVSGLFG RKIGEKGAGI FTSGCLIISL SWSLLIFYET TLNFSTTYIK
LWRWLDSDLV TAYFGLQFDS LTAVMLIVVT SISTLVHIFS TAYMDGDPHV PRFMSYLSLF
TFFMILLVTS DNYPQLFIGW EGVGLCSYLL INFWLTRIEA NKAAIKAMLV NRVGDIGFVL
AMLAIWDQFG CLDFASVFNI VALAPSDNTT LICLFLFIGA VGKSAQLGLH TWLPDAMEGP
TPVSALIHAA TMVTAGVFLL IRSSPLLEQA PMALMVVTIV GSLTAFMAAT IGLVQNDLKK
VIAYSTCSQL GYMVMACGLS QYSISLFHLM NHAFFKALLF LSAGSVIHAL ADEQDMRKMG
GLIRSIPFTY TMIVIGSLSL MGFPYLTGFY SKDLILELAY DQYYLAFAHW LGVFSALLTA
AYSLRLIYLT FISNTNAKKE VFSQAHEGSW NLTLPLILLA LGSIFVGYLT KEIIWSFQIT
LSPIIPTSIK LMPVIFSLFG AGTAVVLYHY SSRIFNAPTS PVGLAGYTFL YSAWQFNYII
NHFLVQNVWR LGHLITFRVL DKGVLELIGP KGISQFMIRL TQEISNLQSG LVYNYALMIL
