NU5M_MOUSE
ID NU5M_MOUSE Reviewed; 607 AA.
AC P03921;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 3.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03915};
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=Mtnd5; Synonyms=mt-Nd5, Nd5;
OS Mus musculus (Mouse).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7332926; DOI=10.1016/0092-8674(81)90300-7;
RA Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A.;
RT "Sequence and gene organization of mouse mitochondrial DNA.";
RL Cell 26:167-180(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=12954771; DOI=10.1093/nar/gkg739;
RA Bayona-Bafaluy M.P., Acin-Perez R., Mullikin J.C., Park J.S.,
RA Moreno-Loshuertos R., Hu P., Perez-Martos A., Fernandez-Silva P., Bai Y.,
RA Enriquez J.A.;
RT "Revisiting the mouse mitochondrial DNA sequence.";
RL Nucleic Acids Res. 31:5349-5355(2003).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03915}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03915};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03920}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03920}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; J01420; AAB48654.1; -; Genomic_DNA.
DR EMBL; V00711; CAA24088.1; -; Genomic_DNA.
DR EMBL; AY172335; AAN85132.1; -; Genomic_DNA.
DR PIR; A00451; QXMS5M.
DR RefSeq; NP_904338.1; NC_005089.1.
DR PDB; 6G2J; EM; 3.30 A; L=1-607.
DR PDB; 6G72; EM; 3.90 A; L=1-607.
DR PDB; 6ZR2; EM; 3.10 A; L=1-607.
DR PDB; 6ZTQ; EM; 3.00 A; L=1-607.
DR PDB; 7AK5; EM; 3.17 A; L=1-607.
DR PDB; 7AK6; EM; 3.82 A; L=1-607.
DR PDB; 7B93; EM; 3.04 A; L=1-607.
DR PDB; 7PSA; EM; 3.40 A; L=1-607.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; P03921; -.
DR SMR; P03921; -.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR IntAct; P03921; 6.
DR MINT; P03921; -.
DR STRING; 10090.ENSMUSP00000081001; -.
DR iPTMnet; P03921; -.
DR PhosphoSitePlus; P03921; -.
DR jPOST; P03921; -.
DR PaxDb; P03921; -.
DR PeptideAtlas; P03921; -.
DR PRIDE; P03921; -.
DR ProteomicsDB; 287844; -.
DR TopDownProteomics; P03921; -.
DR Antibodypedia; 35363; 256 antibodies from 28 providers.
DR Ensembl; ENSMUST00000082418; ENSMUSP00000081001; ENSMUSG00000064367.
DR GeneID; 17721; -.
DR KEGG; mmu:17721; -.
DR CTD; 4540; -.
DR MGI; MGI:102496; mt-Nd5.
DR VEuPathDB; HostDB:ENSMUSG00000064367; -.
DR eggNOG; KOG4668; Eukaryota.
DR GeneTree; ENSGT00730000111303; -.
DR HOGENOM; CLU_007100_6_0_1; -.
DR InParanoid; P03921; -.
DR OMA; GVGIMSF; -.
DR OrthoDB; 526738at2759; -.
DR PhylomeDB; P03921; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR ChiTaRS; mt-Nd5; mouse.
DR PRO; PR:P03921; -.
DR Proteomes; UP000000589; Mitochondrion.
DR RNAct; P03921; protein.
DR Bgee; ENSMUSG00000064367; Expressed in central gray substance of midbrain and 244 other tissues.
DR ExpressionAtlas; P03921; baseline and differential.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0043005; C:neuron projection; ISO:MGI.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IDA:MGI.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0042542; P:response to hydrogen peroxide; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl.
DR GO; GO:0010243; P:response to organonitrogen compound; IEA:Ensembl.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..607
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118114"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 586..606
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 103
FT /note="F -> L (in Ref. 1; AAB48654/CAA24088)"
FT /evidence="ECO:0000305"
FT HELIX 2..22
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 23..25
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 27..30
FT /evidence="ECO:0007829|PDB:6ZR2"
FT HELIX 32..57
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 61..70
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 75..83
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 84..106
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 107..110
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 114..133
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 134..137
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 138..155
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:6G2J"
FT HELIX 164..191
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 198..201
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 202..204
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 205..207
FT /evidence="ECO:0007829|PDB:7AK5"
FT HELIX 211..223
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 236..238
FT /evidence="ECO:0007829|PDB:7B93"
FT HELIX 241..250
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 252..254
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 255..262
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 264..267
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 271..292
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 298..317
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 318..320
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 322..349
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 350..352
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 356..358
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 362..365
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 367..380
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 386..388
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 389..399
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 402..404
FT /evidence="ECO:0007829|PDB:7B93"
FT HELIX 406..429
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 430..432
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 439..441
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 448..471
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 484..487
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 489..506
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 507..509
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 510..512
FT /evidence="ECO:0007829|PDB:7B93"
FT TURN 519..528
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 529..546
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 547..549
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 550..556
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 557..561
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 563..578
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 584..605
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 607 AA; 68475 MW; 31C10862CDC78B10 CRC64;
MNIFTTSILL IFILLLSPIL ISMSNLIKHI NFPLYTTTSI KFSFIISLLP LLMFFHNNME
YMITTWHWVT MNSMELKMSF KTDFFSILFT SVALFVTWSI MQFSSWYMHS DPNINRFIKY
LTLFLITMLI LTSANNMFQL FIGWEGVGIM SFLLIGWWYG RTDANTAALQ AILYNRIGDI
GFILAMVWFS LNMNSWELQQ IMFSNNNDNL IPLMGLLIAA TGKSAQFGLH PWLPSAMEGP
TPVSALLHSS TMVVAGIFLL VRFHPLTTNN NFILTTMLCL GALTTLFTAI CALTQNDIKK
IIAFSTSSQL GLMMVTLGMN QPHLAFLHIC THAFFKAMLF MCSGSIIHSL ADEQDIRKMG
NITKIMPFTS SCLVIGSLAL TGMPFLTGFY SKDLIIEAIN TCNTNAWALL ITLIATSMTA
MYSMRIIYFV TMTKPRFPPL ISINENDPDL MNPIKRLAFG SIFAGFVISY NIPPTSIPVL
TMPWFLKTTA LIISVLGFLI ALELNNLTMK LSMNKANPYS SFSTLLGFFP SIIHRITPMK
SLNLSLKTSL TLLDLIWLEK TIPKSTSTLH TNMTTLTTNQ KGLIKLYFMS FLINIILIII
LYSINLE
