NU5M_OENBE
ID NU5M_OENBE Reviewed; 670 AA.
AC P10330;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=ND5; Synonyms=NAD5;
OS Oenothera berteroana (Bertero's evening primrose).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Onagraceae; Onagroideae; Onagreae; Oenothera.
OX NCBI_TaxID=3950;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Munzia;
RX PubMed=3163767; DOI=10.1007/bf00322444;
RA Wissinger B., Hiesel R., Schuster W., Brennicke A.;
RT "The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains
RT two introns and is co-transcribed with the 5 S rRNA gene.";
RL Mol. Gen. Genet. 212:56-65(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION, AND RNA EDITING.
RX PubMed=1915303; DOI=10.1002/j.1460-2075.1991.tb04912.x;
RA Knoop V., Schuster W., Wissinger B., Brennicke A.;
RT "Trans splicing integrates an exon of 22 nucleotides into the nad5 mRNA in
RT higher plant mitochondria.";
RL EMBO J. 10:3483-3493(1991).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBUNIT: Complex I is composed of about 30 different subunits.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- RNA EDITING: Modified_positions=52 {ECO:0000269|PubMed:1915303}, 81
CC {ECO:0000269|PubMed:1915303}, 91 {ECO:0000269|PubMed:1915303}, 120
CC {ECO:0000269|PubMed:1915303}, 133 {ECO:0000269|PubMed:1915303}, 165
CC {ECO:0000269|PubMed:1915303}, 169 {ECO:0000269|PubMed:1915303}, 180
CC {ECO:0000269|PubMed:1915303}, 183 {ECO:0000269|PubMed:1915303}, 185
CC {ECO:0000269|PubMed:1915303}, 200 {ECO:0000269|PubMed:1915303}, 238
CC {ECO:0000269|PubMed:1915303}, 242 {ECO:0000269|PubMed:1915303}, 279
CC {ECO:0000269|PubMed:1915303}, 288 {ECO:0000269|PubMed:1915303}, 292
CC {ECO:0000269|PubMed:1915303}, 467 {ECO:0000269|PubMed:1915303}, 497
CC {ECO:0000269|PubMed:1915303}, 498 {ECO:0000269|PubMed:1915303}, 517
CC {ECO:0000269|PubMed:1915303}, 523 {ECO:0000269|PubMed:1915303}, 527
CC {ECO:0000269|PubMed:1915303}, 530 {ECO:0000269|PubMed:1915303}, 537
CC {ECO:0000269|PubMed:1915303}, 639 {ECO:0000269|PubMed:1915303}, 640
CC {ECO:0000269|PubMed:1915303};
CC -!- MISCELLANEOUS: Exons a and b and d and e are cis-spliced, while trans-
CC splicing reactions are required to link exons b and c and c and d.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; X07566; CAA30448.3; -; Genomic_DNA.
DR EMBL; X60046; CAA30448.3; JOINED; mRNA.
DR EMBL; X60049; CAA30448.3; JOINED; mRNA.
DR AlphaFoldDB; P10330; -.
DR SMR; P10330; -.
DR PRIDE; P10330; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IEA:UniProt.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 2: Evidence at transcript level;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; RNA editing; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..670
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118119"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 619..639
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 670 AA; 74584 MW; 145D9DEFE96B8AF3 CRC64;
MYLLIVFLPL LGSGVAGFFG RFLGSEGSAI MTTTCVSFSS ILSLIAFYEV ALGASACYLK
IAPWISSEMF DASWGFVFDS LTVVMLIVVT FISSLVHLYS ISYMSEDPHS PRFMCYLSIF
TFFMPMLVTG DNFLQLFLGW EGVGLASYLL IHFWFTRLQA DKAAIKAMLV NRVGDFGLAL
GILGCFTLFQ TVDFSTIFAC ASAPRNSWIS CNMRLNAITL ICILLLIGAV GKSAQIGLHT
WLPDAMEGPT PVSALIHAAT MVTAGVFMIA RCSPLFEYSP TALIVITFAG AMTSFLAATT
GILQNDLKRV IAYSTCSQLG YMIFACGISN YSVSVFHLMN HAFFKALLFL SAGSVIHAMS
DEQDMRKMGG LASSFPFTYA MMLMGSLSLI GFPFLTGFYS KDVILELAYT KYTISGNFAF
WLGSVSVLFT SYYSFRLLFL TFLVPTNSFG RDILRCHDAP IPMAIPLILL ALGSLFVGYL
AKDMMIGLGT NFWANSLFVL PKNEILAESE FAAPTIIKLI PILFSTLGAF LAYNVNLVAD
QFQRAFQTST FCNRLYSFFN KRWFFDQVLN DFLVRSFLRF GYEVSFEALD KGAIEILGPY
GISYTFRRLA ERISQLQSGF VYHYAFAMLL GLTLFVTFFC MWDSLSSWID NRSSFIWIVS
SFYNKKSSQQ
