NU5M_PIG
ID NU5M_PIG Reviewed; 606 AA.
AC Q9TDR1; Q9G7T6;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03915};
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=MT-ND5; Synonyms=MTND5, NADH5, ND5;
OS Sus scrofa (Pig).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Landrace;
RX PubMed=10433971; DOI=10.1016/s0378-1119(99)00247-4;
RA Lin C.S., Sun Y.L., Liu C.Y., Yang P.C., Chang L.C., Cheng I.C.,
RA Mao S.J.T., Huang M.C.;
RT "Complete nucleotide sequence of pig (Sus scrofa) mitochondrial genome and
RT dating evolutionary divergence within artiodactyla.";
RL Gene 236:107-114(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Meishan;
RX PubMed=11428467; DOI=10.1007/s002390010158;
RA Kijas J.M.H., Andersson L.;
RT "A phylogenetic study of the origin of the domestic pig estimated from the
RT near-complete mtDNA genome.";
RL J. Mol. Evol. 52:302-308(2001).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03915}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03915};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03920}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03920}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; AF034253; AAD34195.1; -; Genomic_DNA.
DR EMBL; AF304200; AAG28187.1; -; Genomic_DNA.
DR PIR; T10982; T10982.
DR RefSeq; NP_008644.1; NC_000845.1.
DR PDB; 5GPN; EM; 5.40 A; j=1-606.
DR PDB; 5GUP; EM; 4.00 A; l=1-606.
DR PDBsum; 5GPN; -.
DR PDBsum; 5GUP; -.
DR AlphaFoldDB; Q9TDR1; -.
DR SMR; Q9TDR1; -.
DR STRING; 9823.ENSSSCP00000019145; -.
DR PaxDb; Q9TDR1; -.
DR PeptideAtlas; Q9TDR1; -.
DR PRIDE; Q9TDR1; -.
DR Ensembl; ENSSSCT00000019686; ENSSSCP00000019145; ENSSSCG00000018091.
DR Ensembl; ENSSSCT00070061688; ENSSSCP00070052588; ENSSSCG00070030651.
DR GeneID; 808511; -.
DR KEGG; ssc:808511; -.
DR CTD; 4540; -.
DR VGNC; VGNC:99796; MT-ND5.
DR eggNOG; KOG4668; Eukaryota.
DR GeneTree; ENSGT00730000111303; -.
DR HOGENOM; CLU_007100_6_0_1; -.
DR InParanoid; Q9TDR1; -.
DR OMA; GVGIMSF; -.
DR OrthoDB; 526738at2759; -.
DR TreeFam; TF342974; -.
DR Reactome; R-SSC-611105; Respiratory electron transport.
DR Reactome; R-SSC-6799198; Complex I biogenesis.
DR Proteomes; UP000008227; Mitochondrion.
DR Proteomes; UP000314985; Mitochondrion.
DR Bgee; ENSSSCG00000018091; Expressed in Ammon's horn and 45 other tissues.
DR Genevisible; Q9TDR1; SS.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR010934; NADH_DH_su5_C.
DR InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF06455; NADH5_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..606
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118134"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 582..602
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 13
FT /note="I -> T (in Ref. 2; AAG28187)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="V -> L (in Ref. 2; AAG28187)"
FT /evidence="ECO:0000305"
FT CONFLICT 198
FT /note="L -> F (in Ref. 2; AAG28187)"
FT /evidence="ECO:0000305"
FT CONFLICT 399
FT /note="V -> A (in Ref. 2; AAG28187)"
FT /evidence="ECO:0000305"
FT CONFLICT 434
FT /note="K -> Q (in Ref. 2; AAG28187)"
FT /evidence="ECO:0000305"
FT CONFLICT 600
FT /note="T -> M (in Ref. 2; AAG28187)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 606 AA; 68650 MW; F5D6C256A241D284 CRC64;
MNPFASLTLT TLIILTIPIM MSNSNIYKTN LYPNYVKTTV SYAFTLSLVP LLMFMHTGQE
MIISNWHWMT LQTVELSLSF KMDYFSVMFI PVALFVTWSI MEFSMWYMHS DPFINRFFKY
LVLFLITMMI LVTANNLFQL FIGWEGVGIM SFLLIGWWHG RTDANTAALQ AILYNRIGDI
GFVLSMAWFL THSNAWDLQQ IFMLNNECPN MPLIGLLLAA AGKSAQFGLH PWLPSAMEGP
TPVSALLHSS TMVVAGVFLL IRFYPLMETN KLVQTMTLCL GAITTLFTAL CAITQNDIKK
IVAFSTSSQL GLMMVTIGIN QPHLAFLHIC MHAFFKAMLF MCSGSIIHSL NDEQDIRKMG
GLYKAMPFTT TALIIGSLAL TGMPYLTGFY SKDLIIEAVN MSYTNAWALL MTLIATSLTA
AYSTRIIFFA FLGKPRFPPL VLINENNPLL INSIKRLLIG SIFAGFIISN NIPPMTVPNT
TMPLYMKMTA LIVTIMGFML ALELNNTTYY LKFKYPSQTY KFSNMLGYYP SIMHRLPTYH
NLSMSQKSAS SLLDLIWLET ILPKTTSFIQ MKMSIMVSNQ KGLIKLYFLS FLITIMISMT
LFNYHE
