ID   NU5M_RHIST              Reviewed;         654 AA.
AC   P50367;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 5;
GN   Name=ND5; Synonyms=NAD5;
OS   Rhizopus stolonifer (Rhizopus nigricans).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC   Mucoromycetes; Mucorales; Mucorineae; Rhizopodaceae; Rhizopus.
OX   NCBI_TaxID=4846;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DAOM 148428;
RA   Paquin B., Roewer I., Wang Z., Lang B.F.;
RT   "A robust fungal phylogeny using the mitochondrially encoded nad5 protein
RT   sequence.";
RL   Can. J. Bot. 73:S180-S185(1995).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR   EMBL; U17011; AAA99062.1; -; Genomic_DNA.
DR   PIR; T14202; T14202.
DR   AlphaFoldDB; P50367; -.
DR   SMR; P50367; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR010934; NADH_DH_su5_C.
DR   InterPro; IPR018393; NADHpl_OxRdtase_5_subgr.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   InterPro; IPR003945; NU5C-like.
DR   InterPro; IPR001516; Proton_antipo_N.
DR   PANTHER; PTHR42829; PTHR42829; 1.
DR   Pfam; PF06455; NADH5_C; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   Pfam; PF00662; Proton_antipo_N; 1.
DR   TIGRFAMs; TIGR01974; NDH_I_L; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..654
FT                   /note="NADH-ubiquinone oxidoreductase chain 5"
FT                   /id="PRO_0000118143"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        301..320
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..346
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        365..385
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        406..426
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        451..471
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        510..530
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        612..632
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   654 AA;  72819 MW;  22D2E025B8D6E12D CRC64;
     MYLAILTLPL LSATVAGFLG RKIGKTGSHL ITCSSLVLTA LLALVAFYEV GLCGSPVSIK
     LMSWIDSEFL LVSWGFIYDS LTVSMLLPVL IVSALVHIYS TNYMSEDPHN QRFFAYLSMF
     TFFMLMLVTG DNYLVMFIGW EGVGISSYLL INFWFTRLQA NKAAIKALVM NRVGDWGFSI
     GLWAIFWTFG NLDFTTVFSL APFINEELIT IISICLLVAA MGKSAQIGLH TWLPDAMEGP
     TPVSALIHAA TMVTAGVYLL LRSSPILEFG STALILITWV GALTAFFAAT TGLLQNDLKR
     VIAYSTCSQL GLLFLVCGLS QYNVALFHLV NHAWFKALLF LSAGSVIHAM NDEQDLRKFG
     GLSRLLPFTY SMMVIGSLSL MALPFLTGFY SKDLIIELAY GHYSFSGNLV YWLASVAAVF
     TAMYSIRSLY LTFLGYPNGP KINYNNIHEA PLIMAIPLVV LAVFSIFFGY VTKDLFVGMG
     TDFYNNALFI HPNHSILVDT EFGLPMSMKF LPLIGSLLGT FGVLAIYWIF DELPNKFIST
     KLGRGIYRFF NQKYYFDNIY NNLLNKFLNF GYTTNKILDR GAIELVGPYG LVNVFKSASN
     KVSGLDSGFI PTYAMYIFNG LILFITLIFF IGDPRLFVLL LWAVFLLPNN TTQK