NU5M_TRYBB
ID NU5M_TRYBB Reviewed; 590 AA.
AC P04540;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 5;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 5;
GN Name=ND5;
OS Trypanosoma brucei brucei.
OG Mitochondrion.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=5702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6093040; DOI=10.1093/nar/12.19.7327;
RA Hensgens L.A.M., Brakenhoff J., de Vries B.F., Sloof P., Tromp M.C.,
RA van Boom J.H., Benne R.;
RT "The sequence of the gene for cytochrome c oxidase subunit I, a frameshift
RT containing gene for cytochrome c oxidase subunit II and seven unassigned
RT reading frames in Trypanosoma brucei mitochondrial maxi-circle DNA.";
RL Nucleic Acids Res. 12:7327-7344(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=EATRO 164;
RX PubMed=2437452; DOI=10.1016/0166-6851(87)90057-0;
RA Jasmer D.P., Feagin J.E., Payne M., Stuart K.;
RT "Variation of G-rich mitochondrial transcripts among stocks of Trypanosoma
RT brucei.";
RL Mol. Biochem. Parasitol. 22:259-272(1987).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; X01094; CAB57807.1; -; Genomic_DNA.
DR EMBL; M14820; AAB59225.1; -; Genomic_DNA.
DR PIR; A04519; QQUTC5.
DR AlphaFoldDB; P04540; -.
DR SMR; P04540; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..590
FT /note="NADH-ubiquinone oxidoreductase chain 5"
FT /id="PRO_0000118159"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 501..521
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 536..556
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 568..588
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 590 AA; 71495 MW; CAE231AD9DFF5282 CRC64;
MFLIFFLFFI MFGFISGSFM FGRNFLSFWL SLVMIIFIVL CMIFSFLMVS VCLYGYYYYD
FCLILMLDFC FIWLTYVCSG FYMFIMLLIN MVFCFIVFYA FYYMYFDMLL GRFLIIFWIF
VVCMNLFILS YDFLTAYCGW ELLGLFSFFL ISYFWYRFFA LKFGFKAFFI GKIGDVLLIF
AFSIIFLSNG FCMTTFYFLN FFCMDYYYIE FSICLLVGCA FTKSTQFGLH IWLPDAMEGP
IPVSALIHAA TLVVCGIILL SFVYWCFDFW FSYFYNLIGW STLILILMTL CVFYNFDVKR
YVAFSTICQI SFSMFCCLCI DIYIGSLFFC YHMFYKATLF IVLGIWIHIF FGLQDLRCYF
FMYFCGCVLA RLLLIFAILN SCSIWFLCGF YCKDMLLALL MLLSFYNIIE FLFISIIFIF
FTMIYNYFLL FFLMFVFKCF CLVDCLFLLF DYECCLVYCL ISLYMCILSI FFIIDFVCIF
VFSSYCVFWS FFLNFYNFFD IAIFVVFLIL SVGFLYYGCL FFYFFNIDCI MLFWRIFFVI
IILVVFMIFC CWYFVCMIIF MLLFVWNFVI YFRYNLKYCL FFCILWILYV
