NUBP1_CAEEL
ID NUBP1_CAEEL Reviewed; 313 AA.
AC Q93459;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 17-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Cytosolic Fe-S cluster assembly factor NUBP1 homolog {ECO:0000255|HAMAP-Rule:MF_03038};
DE AltName: Full=Nucleotide binding protein 1 homolog {ECO:0000312|WormBase:F10G8.6};
GN Name=nubp-1 {ECO:0000312|WormBase:F10G8.6};
GN ORFNames=F10G8.6 {ECO:0000312|WormBase:F10G8.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23807208; DOI=10.1007/s00018-013-1401-6;
RA Kypri E., Christodoulou A., Maimaris G., Lethan M., Markaki M.,
RA Lysandrou C., Lederer C.W., Tavernarakis N., Geimer S., Pedersen L.B.,
RA Santama N.;
RT "The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of
RT ciliogenesis.";
RL Cell. Mol. Life Sci. 71:517-538(2014).
CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein
CC assembly (CIA) machinery. Required for maturation of extramitochondrial
CC Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold
CC complex, mediating the de novo assembly of an Fe-S cluster and its
CC transfer to target apoproteins (By similarity). Regulates cilium
CC formation and structure (PubMed:23807208). {ECO:0000255|HAMAP-
CC Rule:MF_03038, ECO:0000269|PubMed:23807208}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03038};
CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable
CC clusters in the N-termini of NUBP1 and two labile, bridging clusters
CC between subunits of the NUBP1-NUBP2 heterotetramer. {ECO:0000255|HAMAP-
CC Rule:MF_03038};
CC -!- SUBUNIT: Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.
CC {ECO:0000255|HAMAP-Rule:MF_03038}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03038}. Cell
CC projection {ECO:0000269|PubMed:23807208}.
CC -!- TISSUE SPECIFICITY: Expressed in head amphid and labial ciliated
CC sensory neurons and tail phasmid ciliated chemosensory neurons.
CC {ECO:0000269|PubMed:23807208}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in abnormal cilia
CC morphology with the formation of 'wing-shaped' cilia structures and
CC additional dendritic ciliated endings in amphid wing B (AWB) olfactory
CC amphid neurons. {ECO:0000269|PubMed:23807208}.
CC -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family.
CC NUBP1/NBP35 subfamily. {ECO:0000255|HAMAP-Rule:MF_03038}.
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DR EMBL; Z80216; CAB02285.2; -; Genomic_DNA.
DR PIR; T20728; T20728.
DR RefSeq; NP_492653.2; NM_060252.4.
DR AlphaFoldDB; Q93459; -.
DR SMR; Q93459; -.
DR BioGRID; 38289; 3.
DR IntAct; Q93459; 1.
DR STRING; 6239.F10G8.6; -.
DR iPTMnet; Q93459; -.
DR EPD; Q93459; -.
DR PaxDb; Q93459; -.
DR PeptideAtlas; Q93459; -.
DR EnsemblMetazoa; F10G8.6.1; F10G8.6.1; WBGene00008664.
DR GeneID; 172868; -.
DR KEGG; cel:CELE_F10G8.6; -.
DR UCSC; F10G8.6; c. elegans.
DR CTD; 172868; -.
DR WormBase; F10G8.6; CE30749; WBGene00008664; nubp-1.
DR eggNOG; KOG3022; Eukaryota.
DR GeneTree; ENSGT00950000183193; -.
DR HOGENOM; CLU_024839_0_1_1; -.
DR InParanoid; Q93459; -.
DR OMA; QHITFKD; -.
DR OrthoDB; 1166096at2759; -.
DR PhylomeDB; Q93459; -.
DR PRO; PR:Q93459; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00008664; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005929; C:cilium; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140663; F:ATP-dependent FeS chaperone activity; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR GO; GO:1902855; P:regulation of non-motile cilium assembly; IMP:WormBase.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_02040; Mrp_NBP35; 1.
DR HAMAP; MF_03038; NUBP1; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR019591; Mrp/NBP35_ATP-bd.
DR InterPro; IPR000808; Mrp_CS.
DR InterPro; IPR028601; NUBP1/Nbp35.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033756; YlxH/NBP35.
DR PANTHER; PTHR23264; PTHR23264; 1.
DR Pfam; PF10609; ParA; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS01215; MRP; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; ATP-binding; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Cytoplasm; Iron; Iron-sulfur; Metal-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..313
FT /note="Cytosolic Fe-S cluster assembly factor NUBP1
FT homolog"
FT /id="PRO_0000184949"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 12
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
FT BINDING 26
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
FT BINDING 29
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
FT BINDING 35
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
FT BINDING 66..73
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
FT BINDING 240
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /ligand_note="ligand shared with heterodimeric partner"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
FT BINDING 243
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /ligand_note="ligand shared with heterodimeric partner"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03038"
SQ SEQUENCE 313 AA; 33038 MW; 471C5D7409D6661F CRC64;
MSDVPDDANA GCPGTGSAGA GKASGCAGCP NQGSCATGQG PPPDADVPKI QDRFSRIKHK
ILILSGKGGV GKSTLTSNLA RALASDPSKQ VAILDVDICG PSQPRMMGVE DEEVHNSADG
WTPVGIQPNL TLMSIAFLLG DKNDAVIWRG ARKNGMIKQF LKDVDWGEVD YLLIDTPPGT
SDEHISLVQF LLQAGPLDGA LIVSTPQEVS LLDVRKEVSF CVKTKVPILG VVENMARFVC
PNCAHTTLLF PTSTGGAEQM CKDSNLELLA QLPLEPALAK ALDNGEDFFE TNPDSTLAKS
FLDLAEKVKA KLV
