NUBP2_XENTR
ID NUBP2_XENTR Reviewed; 270 AA.
AC A4QNM5;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Cytosolic Fe-S cluster assembly factor nubp2 {ECO:0000255|HAMAP-Rule:MF_03039};
DE AltName: Full=Nucleotide-binding protein 2 {ECO:0000255|HAMAP-Rule:MF_03039};
DE Short=NBP 2 {ECO:0000255|HAMAP-Rule:MF_03039};
GN Name=nubp2;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Richardson P., Lucas S., Rokhsar D., Detter J.C., Ng D.C., Brokstein P.,
RA Lindquist E.A.;
RT "DOE Joint Genome Institute Xenopus tropicalis EST project.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein
CC assembly (CIA) machinery. Required for maturation of extramitochondrial
CC Fe-S proteins. The nubp1-nubp2 heterotetramer forms a Fe-S scaffold
CC complex, mediating the de novo assembly of an Fe-S cluster and its
CC transfer to target apoproteins. {ECO:0000255|HAMAP-Rule:MF_03039}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03039};
CC Note=Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable
CC clusters in the N-termini of nubp1 and two labile, bridging clusters
CC between subunits of the nubp1-nubp2 heterotetramer. {ECO:0000255|HAMAP-
CC Rule:MF_03039};
CC -!- SUBUNIT: Heterotetramer of 2 nubp1 and 2 nubp2 chains.
CC {ECO:0000255|HAMAP-Rule:MF_03039}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03039}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A4QNM5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A4QNM5-2; Sequence=VSP_037923;
CC -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family.
CC NUBP2/CFD1 subfamily. {ECO:0000255|HAMAP-Rule:MF_03039}.
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DR EMBL; CX932505; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC135883; AAI35884.1; -; mRNA.
DR RefSeq; NP_001096534.1; NM_001103064.1. [A4QNM5-2]
DR RefSeq; XP_012825448.1; XM_012969994.2. [A4QNM5-1]
DR AlphaFoldDB; A4QNM5; -.
DR SMR; A4QNM5; -.
DR STRING; 8364.ENSXETP00000002486; -.
DR PaxDb; A4QNM5; -.
DR GeneID; 100125178; -.
DR KEGG; xtr:100125178; -.
DR CTD; 10101; -.
DR Xenbase; XB-GENE-999229; nubp2.
DR eggNOG; KOG3022; Eukaryota.
DR HOGENOM; CLU_024839_0_1_1; -.
DR InParanoid; A4QNM5; -.
DR OMA; EEGHHFI; -.
DR OrthoDB; 1166096at2759; -.
DR PhylomeDB; A4QNM5; -.
DR TreeFam; TF354321; -.
DR Proteomes; UP000008143; Chromosome 9.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000010354; Expressed in mesonephros and 13 other tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140663; F:ATP-dependent FeS chaperone activity; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_02040; Mrp_NBP35; 1.
DR HAMAP; MF_03039; NUBP2; 1.
DR InterPro; IPR019591; Mrp/NBP35_ATP-bd.
DR InterPro; IPR000808; Mrp_CS.
DR InterPro; IPR028600; NUBP2/Cfd1_eukaryotes.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033756; YlxH/NBP35.
DR PANTHER; PTHR23264; PTHR23264; 1.
DR Pfam; PF10609; ParA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS01215; MRP; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Alternative splicing; ATP-binding; Cytoplasm; Iron; Iron-sulfur;
KW Metal-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..270
FT /note="Cytosolic Fe-S cluster assembly factor nubp2"
FT /id="PRO_0000382704"
FT BINDING 22..29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03039"
FT BINDING 196
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03039"
FT BINDING 199
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03039"
FT VAR_SEQ 164..200
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_037923"
SQ SEQUENCE 270 AA; 28910 MW; 88C8C44F0A454CB6 CRC64;
MERSQDGGNL SGVQHIILVL SGKGGVGKST ISTEIALALR HAGKKVGILD VDLCGPSIPR
MLNAQSKDVH QCDSGWVPVY VDQEKSISLM SIGFLLEHPD DAVVWRGPKK NALIKQFASD
VAWGDLDFLI VDTPPGTSDE HIATVDALRP FNPMGALLVT TPQAVSVGDV RRELTFCKKT
GLRVIGIVEN MSGYVCPHCT ECTNIFSKGG GEELARLSGV PFLGCVPLDP LLSQSLEQGK
DFVQEFPNSA AYPAISSIAR QILDMASPRS
