ID   NUBPL_DICDI             Reviewed;         323 AA.
AC   Q54F15;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Iron-sulfur protein NUBPL;
DE   AltName: Full=Nucleotide-binding protein-like;
DE   Flags: Precursor;
GN   Name=nubpl; ORFNames=DDB_G0291193;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Required for the assembly of the mitochondrial membrane
CC       respiratory chain NADH dehydrogenase (Complex I). May deliver of one or
CC       more Fe-S clusters to complex I subunits (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000175; EAL61880.1; -; Genomic_DNA.
DR   RefSeq; XP_635375.1; XM_630283.1.
DR   AlphaFoldDB; Q54F15; -.
DR   SMR; Q54F15; -.
DR   STRING; 44689.DDB0232425; -.
DR   PaxDb; Q54F15; -.
DR   EnsemblProtists; EAL61880; EAL61880; DDB_G0291193.
DR   GeneID; 8628023; -.
DR   KEGG; ddi:DDB_G0291193; -.
DR   dictyBase; DDB_G0291193; nubpl.
DR   eggNOG; KOG3022; Eukaryota.
DR   HOGENOM; CLU_024839_0_2_1; -.
DR   InParanoid; Q54F15; -.
DR   OMA; NMAYFTP; -.
DR   PhylomeDB; Q54F15; -.
DR   Reactome; R-DDI-6799198; Complex I biogenesis.
DR   PRO; PR:Q54F15; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140663; F:ATP-dependent FeS chaperone activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_02040; Mrp_NBP35; 1.
DR   InterPro; IPR019591; Mrp/NBP35_ATP-bd.
DR   InterPro; IPR044304; NUBPL-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033756; YlxH/NBP35.
DR   PANTHER; PTHR42961; PTHR42961; 1.
DR   Pfam; PF10609; ParA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Iron; Iron-sulfur; Metal-binding; Mitochondrion;
KW   Nucleotide-binding; Reference proteome; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           ?..323
FT                   /note="Iron-sulfur protein NUBPL"
FT                   /id="PRO_0000327673"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   323 AA;  35479 MW;  1EA0BAE389554B1E CRC64;
     MFKKLITTPF FSPNKQFITF QLESGKRNYF SNNKIQLHGG SGHRQPQVTK VAIEGIKNII
     AVSSAKGGVG KSTCAVNIAL GLSSHNLSVG LLDVDVFGPS IPLMMDLKNH EKPFTNELNQ
     MIPLQNYGIK CMSMGFLVNE DDPIIWRGPM VGSALEKLLR QTDWGHLDVL VCDLPPGTGD
     AILTMCQRVP LTGAVIVSTP QDVALADVVR GVNMFKKVEV PILGLVENMS YFNCPHCNES
     THIFGNEGAK NTAKKMGINF LGDVPIHLQI RETSDSGKPI TVTQPDSPQA KNYKDISKEI
     IKQLEIINND ENKDNKEPNI IIT