ID   NUBPL_MOUSE             Reviewed;         319 AA.
AC   Q9CWD8; Q6P6K2;
DT   07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Iron-sulfur protein NUBPL;
DE   AltName: Full=Nucleotide-binding protein-like;
DE   Flags: Precursor;
GN   Name=Nubpl;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Embryonic stem cell, and Head;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Required for the assembly of the mitochondrial membrane
CC       respiratory chain NADH dehydrogenase (Complex I). May deliver of one or
CC       more Fe-S clusters to complex I subunits (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mrp/NBP35 ATP-binding proteins family.
CC       {ECO:0000305}.
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DR   EMBL; AK010827; BAB27209.1; -; mRNA.
DR   EMBL; AK152377; BAE31166.1; -; mRNA.
DR   EMBL; BC062175; AAH62175.1; -; mRNA.
DR   CCDS; CCDS36442.2; -.
DR   RefSeq; NP_084036.2; NM_029760.2.
DR   AlphaFoldDB; Q9CWD8; -.
DR   SMR; Q9CWD8; -.
DR   IntAct; Q9CWD8; 1.
DR   STRING; 10090.ENSMUSP00000044292; -.
DR   iPTMnet; Q9CWD8; -.
DR   PhosphoSitePlus; Q9CWD8; -.
DR   EPD; Q9CWD8; -.
DR   jPOST; Q9CWD8; -.
DR   MaxQB; Q9CWD8; -.
DR   PaxDb; Q9CWD8; -.
DR   PeptideAtlas; Q9CWD8; -.
DR   PRIDE; Q9CWD8; -.
DR   ProteomicsDB; 293805; -.
DR   Antibodypedia; 23093; 264 antibodies from 23 providers.
DR   Ensembl; ENSMUST00000040090; ENSMUSP00000044292; ENSMUSG00000035142.
DR   GeneID; 76826; -.
DR   KEGG; mmu:76826; -.
DR   UCSC; uc007nnh.2; mouse.
DR   CTD; 80224; -.
DR   MGI; MGI:1924076; Nubpl.
DR   VEuPathDB; HostDB:ENSMUSG00000035142; -.
DR   eggNOG; KOG3022; Eukaryota.
DR   GeneTree; ENSGT00950000183193; -.
DR   HOGENOM; CLU_024839_0_2_1; -.
DR   InParanoid; Q9CWD8; -.
DR   OMA; NMAYFTP; -.
DR   OrthoDB; 1166096at2759; -.
DR   PhylomeDB; Q9CWD8; -.
DR   TreeFam; TF323196; -.
DR   Reactome; R-MMU-6799198; Complex I biogenesis.
DR   BioGRID-ORCS; 76826; 11 hits in 69 CRISPR screens.
DR   ChiTaRS; Nubpl; mouse.
DR   PRO; PR:Q9CWD8; -.
DR   Proteomes; UP000000589; Chromosome 12.
DR   RNAct; Q9CWD8; protein.
DR   Bgee; ENSMUSG00000035142; Expressed in heart left ventricle and 72 other tissues.
DR   ExpressionAtlas; Q9CWD8; baseline and differential.
DR   Genevisible; Q9CWD8; MM.
DR   GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; ISO:MGI.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140663; F:ATP-dependent FeS chaperone activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IBA:GO_Central.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISO:MGI.
DR   GO; GO:0070584; P:mitochondrion morphogenesis; ISO:MGI.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_02040; Mrp_NBP35; 1.
DR   InterPro; IPR019591; Mrp/NBP35_ATP-bd.
DR   InterPro; IPR000808; Mrp_CS.
DR   InterPro; IPR044304; NUBPL-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033756; YlxH/NBP35.
DR   PANTHER; PTHR42961; PTHR42961; 1.
DR   Pfam; PF10609; ParA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS01215; MRP; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Iron; Iron-sulfur; Metal-binding; Mitochondrion;
KW   Nucleotide-binding; Reference proteome; Transit peptide.
FT   TRANSIT         1..38
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..319
FT                   /note="Iron-sulfur protein NUBPL"
FT                   /id="PRO_0000184951"
FT   BINDING         75..82
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        292
FT                   /note="F -> L (in Ref. 1; BAB27209)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   319 AA;  34139 MW;  5455BB7F2F1F8713 CRC64;
     MGTWRRLLLF GGVSLRGGGA ATVPPRGCRA LGCGRQLLGA ESEALKQRRT QIMSRGLPKQ
     KPIEGVREVI VVASGKGGVG KSTTAVNLAL ALAANDSSKA VGLLDVDVYG PSIPKMMNLR
     GNPELSPNNL MRPLLNYGIA CMSMGFLVEE TAPLVWRGLM VMSAIEKLLR QVDWGQLDYL
     VVDMPPGTGD VQLSVSQNIP ISGAVIVSTP QDIALMDAHK GAEMFRKVNV PVLGLVQNMS
     VFQCPKCKHK THIFGADGAR KLAQTLDLDV LGDVPLHLSI REASDMGQPV VFSQPGSDEA
     KAYLHIASEV VRRLKSSPE