ID   NUC2_NEUCR              Reviewed;        1066 AA.
AC   Q01317; A7UXH8; Q7RW36;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   09-MAY-2003, sequence version 2.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Ankyrin repeat protein nuc-2;
DE   AltName: Full=Nuclease 2;
GN   Name=nuc-2; ORFNames=B23G1.010, NCU01847, NCU11426;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=74-OR23-1VA / FGSC 2489;
RX   PubMed=8917314; DOI=10.1007/bf02173977;
RA   Poleg Y., Aramayo R., Kang S., Hall J.G., Metzenberg R.L.;
RT   "NUC-2, a component of the phosphate-regulated signal transduction pathway
RT   in Neurospora crassa, is an ankyrin repeat protein.";
RL   Mol. Gen. Genet. 252:709-716(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Controls phosphorus acquisition.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDO64869.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; U51118; AAB03277.1; -; Genomic_DNA.
DR   EMBL; BX284754; CAD70463.1; -; Genomic_DNA.
DR   EMBL; CM002237; EDO64869.2; ALT_SEQ; Genomic_DNA.
DR   PIR; S72479; S72479.
DR   RefSeq; XP_001727960.2; XM_001727908.2.
DR   AlphaFoldDB; Q01317; -.
DR   SMR; Q01317; -.
DR   STRING; 5141.EFNCRP00000009831; -.
DR   EnsemblFungi; EDO64869; EDO64869; NCU11426.
DR   GeneID; 5846981; -.
DR   KEGG; ncr:NCU11426; -.
DR   HOGENOM; CLU_002842_1_0_1; -.
DR   InParanoid; Q01317; -.
DR   Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 1.25.40.20; -; 1.
DR   Gene3D; 3.20.20.190; -; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR030395; GP_PDE_dom.
DR   InterPro; IPR001995; Peptidase_A2_cat.
DR   InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR   InterPro; IPR004331; SPX_dom.
DR   Pfam; PF12796; Ank_2; 2.
DR   Pfam; PF13637; Ank_4; 1.
DR   Pfam; PF03009; GDPD; 1.
DR   Pfam; PF03105; SPX; 1.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 9.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   SUPFAM; SSF51695; SSF51695; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 4.
DR   PROSITE; PS51704; GP_PDE; 1.
DR   PROSITE; PS51382; SPX; 1.
PE   4: Predicted;
KW   ANK repeat; Reference proteome; Repeat.
FT   CHAIN           1..1066
FT                   /note="Ankyrin repeat protein nuc-2"
FT                   /id="PRO_0000067064"
FT   DOMAIN          1..166
FT                   /note="SPX"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT   REPEAT          268..298
FT                   /note="ANK 1"
FT   REPEAT          336..366
FT                   /note="ANK 2"
FT   REPEAT          370..399
FT                   /note="ANK 3"
FT   REPEAT          403..432
FT                   /note="ANK 4"
FT   REPEAT          435..465
FT                   /note="ANK 5"
FT   REPEAT          470..499
FT                   /note="ANK 6"
FT   REPEAT          503..532
FT                   /note="ANK 7"
FT   DOMAIN          717..1048
FT                   /note="GP-PDE"
FT   REGION          923..963
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        941..963
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        857
FT                   /note="A -> V (in Ref. 1; AAB03277)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1066 AA;  117115 MW;  1E189EA92FB62B75 CRC64;
     MKFGKQIQKR QLEVPEYAAS FVNYKALKKL IKKLSATPIL PPQTDLRRAP GEPLDTQSAL
     QANKATFFFQ IDRELDKVNA CYVQKEAELK IRLKTLLDKK KALRSRSGGT SRRSTKFTTL
     QEGFQQFVND LNKLQQFVEI NGTAFSKILK KWDKTAKSKT KELYLSRVVE KRPAFNPTVI
     SELSDQATTS LQELGAWADG DNVSFETRPD HDHVVSTQHL LGTDEGDADT LLLDTVLSGN
     IDSLKDMLDR MKATAAPDGS ADVSLAERVT RTFLASINEG SLEALKVLLD TGLVDIQSWD
     DINERNCLHQ AAIYGNSFVL EYGLSKGVDA ERTDAYGRVP LHYASMHGRL DMIDALLNAS
     PKTINLIDHD NFTPLVHSIV RNHLECVGRL LERSARIDPV SDTDHVPLNL ACQHGSVAIV
     ELLLKHGAKI LADAEGLYPQ HLVARSGQTP EILVLLKQYG ADLDQIDKLY GWTPLVHAAS
     EGNVPCLQAL LETGADPNIL DEKDLPAMYY AAWEGHLECM KLLTPAKKEK AASELPPIHI
     GGALPPMASS TAPMPMSLDA IDPIPALELP PPIIPLRRYG HNFLDTKTVV QISFEEDSEQ
     PLLFFQDGKY PAARLTISSK SSDLIPKNII LPFQEDTRVA SFQIDNLESF TLDFEVFPTY
     GAKVIAKTVA LPNIFRALLS SSGKCCLPLF DPRLRAIGQI SFHVQIIKPF SGTPLEITDF
     ETYWKATSQF DTNTSTFVTG SSLSGDFVQI YVQHTKDGVP VLWPRWTINC GGIDVPVSTL
     TLAQFQTVTA AARNRINLSE LSTYTLDQIA DVHRILANIG ITLHEALFLL PKGMHVNIQV
     LYPTADEKAE AKSSTAADDV NEFADAILSV VFDHARAQRA ERPDSVRSVV FSSYNPTLCT
     ALNWKQPNFP VFLCNDMGRE DRKQQQQGSC SKGDGDEDMG GTTAASRREA ADERTLQSDG
     RRTSSIKDVV RTATSNNLMG LICCSRLLDM VPALVDAIKS HGLALVVDKS GEPEAANSKD
     SGDKQLGISG ALGGLAGSDP FPKLPKGVDG LLKSNGVLRF NEYIDV