NUCB_BACSU
ID NUCB_BACSU Reviewed; 136 AA.
AC P42983;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Sporulation-specific extracellular nuclease;
DE EC=3.-.-.-;
DE Flags: Precursor;
GN Name=nucB; OrderedLocusNames=BSU25750;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7746143; DOI=10.1111/j.1365-2958.1995.tb02236.x;
RA van Sinderen D., Kiewiet R., Venema G.;
RT "Differential expression of two closely related deoxyribonuclease genes,
RT nucA and nucB, in Bacillus subtilis.";
RL Mol. Microbiol. 15:213-223(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
RX PubMed=2105293; DOI=10.1128/jb.172.2.1092-1098.1990;
RA Sato T., Samori Y., Kobayashi Y.;
RT "The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a
RT protein homologous to a site-specific recombinase.";
RL J. Bacteriol. 172:1092-1098(1990).
CC -!- FUNCTION: Degrades both double-stranded linear and covalently closed
CC circular DNA. Likely to play a scavenging role in order to supply
CC nutrients under starvation conditions.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Note=Mn(2+) ion stimulates activity.;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DEVELOPMENTAL STAGE: Sporulation-specific.
CC -!- SIMILARITY: To B.subtilis NucA/ComI. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; S78249; AAB34262.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12437.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14516.1; -; Genomic_DNA.
DR EMBL; M29040; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S61273; S61273.
DR RefSeq; NP_390452.1; NC_000964.3.
DR RefSeq; WP_009967785.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P42983; -.
DR SMR; P42983; -.
DR STRING; 224308.BSU25750; -.
DR PaxDb; P42983; -.
DR PRIDE; P42983; -.
DR EnsemblBacteria; CAB14516; CAB14516; BSU_25750.
DR GeneID; 937804; -.
DR KEGG; bsu:BSU25750; -.
DR PATRIC; fig|224308.179.peg.2800; -.
DR eggNOG; COG3209; Bacteria.
DR OMA; RYPETGD; -.
DR BioCyc; BSUB:BSU25750-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR029476; DNase_NucA_NucB.
DR Pfam; PF14040; DNase_NucA_NucB; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Manganese; Nuclease; Reference proteome; Secreted; Signal;
KW Sporulation.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..136
FT /note="Sporulation-specific extracellular nuclease"
FT /id="PRO_0000021866"
FT REGION 63..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 136 AA; 14968 MW; C2A9391A22DB0764 CRC64;
MKKWMAGLFL AAAVLLCLMV PQQIQGASSY DKVLYFPLSR YPETGSHIRD AIAEGHPDIC
TIDRDGADKR REESLKGIPT KPGYDRDEWP MAVCEEGGAG ADVRYVTPSD NRGAGSWVGN
QMSSYPDGTR VLFIVQ
