ID   NUCK_BPT7               Reviewed;         196 AA.
AC   P03781;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Nucleotide kinase gp1.7;
DE   AltName: Full=Gene product 1.7;
DE            Short=Gp1.7;
GN   OrderedLocusNames=1.7;
OS   Escherichia phage T7 (Bacteriophage T7).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus.
OX   NCBI_TaxID=10760;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6864790; DOI=10.1016/s0022-2836(83)80282-4;
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of
RT   T7 genetic elements.";
RL   J. Mol. Biol. 166:477-535(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7310871; DOI=10.1016/0022-2836(81)90178-9;
RA   Dunn J.J., Studier F.W.;
RT   "Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to
RT   the beginning of gene 4.";
RL   J. Mol. Biol. 148:303-330(1981).
RN   [3]
RP   FUNCTION.
RX   PubMed=18599435; DOI=10.1073/pnas.0804164105;
RA   Tran N.Q., Rezende L.F., Qimron U., Richardson C.C., Tabor S.;
RT   "Gene 1.7 of bacteriophage T7 confers sensitivity of phage growth to
RT   dideoxythymidine.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:9373-9378(2008).
RN   [4]
RP   FUNCTION, ISOFORM SMALL GP1.7, AND SUBUNIT.
RX   PubMed=20497505; DOI=10.1111/j.1365-2958.2010.07221.x;
RA   Tran N.Q., Lee S.J., Richardson C.C., Tabor S.;
RT   "A novel nucleotide kinase encoded by gene 1.7 of bacteriophage T7.";
RL   Mol. Microbiol. 77:492-504(2010).
RN   [5]
RP   FUNCTION, AND MUTAGENESIS OF TYR-128.
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Nucleotide kinase that catalyzes the phosphorylation of dGMP
CC       and dTMP to dGDP and dTDP (PubMed:18599435, PubMed:20497505). A double
CC       mutation in this protein and the RecBCD inhibitor gp5.9 protein allow
CC       phage to overcome the retron Ec48 bacteriophage defense system. This
CC       protein alone when overexpressed in E.coli does not cause growth
CC       arrest; Y128C may be a silent mutation (PubMed:33157039).
CC       {ECO:0000269|PubMed:18599435, ECO:0000269|PubMed:20497505,
CC       ECO:0000269|PubMed:33157039}.
CC   -!- SUBUNIT: Dodecamer. {ECO:0000269|PubMed:20497505}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=Large gp1.7;
CC         IsoId=P03781-1; Sequence=Displayed;
CC       Name=Small gp1.7;
CC         IsoId=P03781-2; Sequence=VSP_053707;
CC   -!- MISCELLANEOUS: [Isoform Small gp1.7]: Produced by alternative
CC       initiation at Met-42 of isoform Small gp1.7. {ECO:0000305}.
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DR   EMBL; V01146; CAA24397.1; -; Genomic_DNA.
DR   EMBL; V01127; CAA24340.1; -; Genomic_DNA.
DR   PIR; G43002; W1BP77.
DR   RefSeq; NP_041967.1; NC_001604.1. [P03781-1]
DR   MINT; P03781; -.
DR   GeneID; 1261060; -.
DR   KEGG; vg:1261060; -.
DR   BioCyc; MetaCyc:MON-19581; -.
DR   Proteomes; UP000000840; Genome.
DR   InterPro; IPR021739; SaV-like.
DR   Pfam; PF11753; DUF3310; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Late protein; Reference proteome.
FT   CHAIN           1..196
FT                   /note="Nucleotide kinase gp1.7"
FT                   /id="PRO_0000106477"
FT   REGION          97..118
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..41
FT                   /note="Missing (in isoform Small gp1.7)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_053707"
FT   MUTAGEN         128
FT                   /note="Y->C: Allows phage to overcome the retron Ec48
FT                   defense system; when associated with 'P-23' in the gp5.9
FT                   protein."
FT                   /evidence="ECO:0000269|PubMed:33157039"
SQ   SEQUENCE   196 AA;  22184 MW;  A1E2CA26FDB794C6 CRC64;
     MGLLDGEAWE KENPPVQATG CIACLEKDDR YPHTCNKGAN DMTEREQEMI IKLIDNNEGR
     PDDLNGCGIL CSNVPCHLCP ANNDQKITLG EIRAMDPRKP HLNKPEVTPT DDQPSAETIE
     GVTKPSHYML FDDIEAIEVI ARSMTVEQFK GYCFGNILKY RLRAGKKSEL AYLEKDLAKA
     DFYKELFEKH KDKCYA