ID   NUCLP_PAELB             Reviewed;         434 AA.
AC   A0A2A5JY22;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Nucleobase transporter PlUacP {ECO:0000303|PubMed:30087835};
GN   ORFNames=PL1_3014 {ECO:0000312|EMBL:PCK69666.1};
OS   Paenibacillus larvae subsp. larvae (strain NRRL B-3650 / LMG 16245).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=741161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=21923906; DOI=10.1186/1471-2164-12-450;
RA   Chan Q.W., Cornman R.S., Birol I., Liao N.Y., Chan S.K., Docking T.R.,
RA   Jackman S.D., Taylor G.A., Jones S.J., de Graaf D.C., Evans J.D.,
RA   Foster L.J.;
RT   "Updated genome assembly and annotation of Paenibacillus larvae, the agent
RT   of American foulbrood disease of honey bees.";
RL   BMC Genomics 12:450-450(2011).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=30087835; DOI=10.1002/2211-5463.12488;
RA   Stoffer-Bittner A.J., Alexander C.R., Dingman D.W., Mourad G.S.,
RA   Schultes N.P.;
RT   "The solute transport and binding profile of a novel nucleobase cation
RT   symporter 2 from the honeybee pathogen Paenibacillus larvae.";
RL   FEBS Open Bio 8:1322-1331(2018).
CC   -!- FUNCTION: Uptake of the purines adenine and guanine, and the pyrimidine
CC       uracil. Transport is probably proton-dependent.
CC       {ECO:0000269|PubMed:30087835}.
CC   -!- ACTIVITY REGULATION: Inhibited by the proton gradient disruptor
CC       carbonyl cyanide m-chlorophenylhydrazone (CCCP), but not by the sodium
CC       gradient disruptor ouabain. Hypoxanthine, xanthine, cytosine and uric
CC       acid act as competitive inhibitors. {ECO:0000269|PubMed:30087835}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=7.04 uM for adenine {ECO:0000269|PubMed:30087835};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; ADZY03000323; PCK69666.1; -; Genomic_DNA.
DR   RefSeq; WP_023485062.1; NZ_ADZY03000323.1.
DR   AlphaFoldDB; A0A2A5JY22; -.
DR   SMR; A0A2A5JY22; -.
DR   EnsemblBacteria; PCK69666; PCK69666; PL1_3014.
DR   GeneID; 64217211; -.
DR   Proteomes; UP000217691; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:UniProt.
DR   InterPro; IPR006043; NCS2.
DR   InterPro; IPR017588; UacT-like.
DR   InterPro; IPR006042; Xan_ur_permease.
DR   Pfam; PF00860; Xan_ur_permease; 1.
DR   TIGRFAMs; TIGR00801; ncs2; 1.
DR   TIGRFAMs; TIGR03173; pbuX; 1.
DR   PROSITE; PS01116; XANTH_URACIL_PERMASE; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..434
FT                   /note="Nucleobase transporter PlUacP"
FT                   /id="PRO_0000446237"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        365..385
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   434 AA;  45792 MW;  5604C2CDD44CB9E8 CRC64;
     MRKSKVLTLG FQHVLAMYAG AVIVPLIVGS SLKLNAEQLA YLVSIDLLTC GIATLLQVWR
     NKFFGIGLPV MLGCTFTAVG PMIAIGSEYG MPAIYGSVLA SGLFLALFAG FFGKLARFFP
     PIVTGSVVTI IGITLIPVAV QDMGGGQGSA DFGSLSNLAL SFGVLLFIIL ANRFFTGFIR
     AISILLGLIF GTIAGAFMGK VDIGPLLDAS WFHGIHPFYF GFPTFHLPSI LTMTLVAIVS
     VMESTGVFVA LGKITEKELT ADDLKRGYRS EGLASILGSI MNSFPYTTYS QNVGLIQISK
     VKSRDVVITA GFILVILGFM PKIAALTLLI PTAVLGGAMI AMFGMVVSSG IKMLGAIDLN
     NHENLLIIAC SVSVGLGVTV APNLFDHLPD SIKILTSNGI VAGSLTAILM NFLFTVGRKK
     QDESHASAEN VHAA