NUCMR_DICDI
ID NUCMR_DICDI Reviewed; 756 AA.
AC Q9U7C9; C7FZX6; Q55BM2; Q55BM3; Q6GW01;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Nucleomorphin;
DE AltName: Full=Calmodulin-binding protein A;
DE AltName: Full=Calmodulin-binding protein CaM-BP38;
GN Name=numA;
GN Synonyms=cmbA {ECO:0000312|dictyBase:DDB_G0269114},
GN numB {ECO:0000312|EMBL:AAT48090.1}; ORFNames=DDB_G0269114;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAT48090.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CALMODULIN,
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DOMAIN DEED MOTIF, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=11919178; DOI=10.1074/jbc.m109717200;
RA Myre M.A., O'Day D.H.;
RT "Nucleomorphin. A novel, acidic, nuclear calmodulin-binding protein from
RT Dictyostelium that regulates nuclear number.";
RL J. Biol. Chem. 277:19735-19744(2002).
RN [2] {ECO:0000312|EMBL:AAT48090.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND DEVELOPMENTAL STAGE.
RX PubMed=15535983; DOI=10.1016/j.bbagen.2004.08.002;
RA Myre M.A., O'Day D.H.;
RT "Dictyostelium nucleomorphin is a member of the BRCT-domain family of cell
RT cycle checkpoint proteins.";
RL Biochim. Biophys. Acta 1675:192-197(2004).
RN [3] {ECO:0000312|EMBL:EAL71906.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000312|EMBL:EAL71906.1};
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=11923193; DOI=10.1242/dev.129.7.1543;
RA Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M.,
RA Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H.,
RA Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.;
RT "A transcriptional profile of multicellular development in Dictyostelium
RT discoideum.";
RL Development 129:1543-1552(2002).
RN [5] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=12912885; DOI=10.1128/ec.2.4.664-670.2003;
RA Iranfar N., Fuller D., Loomis W.F.;
RT "Genome-wide expression analyses of gene regulation during early
RT development of Dictyostelium discoideum.";
RL Eukaryot. Cell 2:664-670(2003).
RN [6] {ECO:0000305}
RP INTERACTION WITH CBPD1.
RX PubMed=15325281; DOI=10.1016/j.bbrc.2004.07.168;
RA Myre M.A., O'Day D.H.;
RT "Dictyostelium calcium-binding protein 4a interacts with nucleomorphin, a
RT BRCT-domain protein that regulates nuclear number.";
RL Biochem. Biophys. Res. Commun. 322:665-671(2004).
RN [7] {ECO:0000305}
RP NUCLEAR LOCALIZATION SIGNAL, AND SUBCELLULAR LOCATION.
RX PubMed=15896312; DOI=10.1016/j.bbrc.2005.04.101;
RA Myre M.A., O'Day D.H.;
RT "An N-terminal nuclear localization sequence but not the calmodulin-binding
RT domain mediates nuclear localization of nucleomorphin, a protein that
RT regulates nuclear number in Dictyostelium.";
RL Biochem. Biophys. Res. Commun. 332:157-166(2005).
CC -!- SUBUNIT: Interacts with calmodulin and CBPD1 in the presence of Ca(2+).
CC {ECO:0000269|PubMed:11919178, ECO:0000269|PubMed:15325281}.
CC -!- INTERACTION:
CC Q9U7C9; Q54RF4: cbpD1; NbExp=2; IntAct=EBI-530708, EBI-531591;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11919178,
CC ECO:0000269|PubMed:15896312}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=2 {ECO:0000269|PubMed:15535983}; Synonyms=numA2
CC {ECO:0000312|dictyBase:DDB_G0269114}, NumB
CC {ECO:0000269|PubMed:15535983};
CC IsoId=Q9U7C9-1; Sequence=Displayed;
CC Name=1 {ECO:0000269|PubMed:11919178}; Synonyms=numA1
CC {ECO:0000312|dictyBase:DDB_G0269114}, NumA
CC {ECO:0000269|PubMed:11919178};
CC IsoId=Q9U7C9-2; Sequence=VSP_052481;
CC -!- DEVELOPMENTAL STAGE: Isoform 1 is expressed throughout development.
CC Isoform 2 is expressed at low level during vegetative growth.
CC Expression of isoform 2 increases rapidly during aggregation (1-4
CC hours) and remains high throughout development.
CC {ECO:0000269|PubMed:11919178, ECO:0000269|PubMed:11923193,
CC ECO:0000269|PubMed:12912885, ECO:0000269|PubMed:15535983}.
CC -!- DISRUPTION PHENOTYPE: Mutants lacking the DEED domain form
CC multinucleated cells. {ECO:0000269|PubMed:11919178}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEU04155.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF140042; AAD56608.2; -; mRNA.
DR EMBL; AY631855; AAT48090.1; -; mRNA.
DR EMBL; AAFI02000005; EAL71906.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71907.2; -; Genomic_DNA.
DR EMBL; AAFI02000005; EEU04155.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001134480.1; XM_001134480.1.
DR RefSeq; XP_002649205.1; XM_002649159.1.
DR RefSeq; XP_646808.2; XM_641716.2.
DR AlphaFoldDB; Q9U7C9; -.
DR IntAct; Q9U7C9; 1.
DR STRING; 44689.DDB0231257; -.
DR PaxDb; Q9U7C9; -.
DR EnsemblProtists; EAL71906; EAL71906; DDB_G0269114.
DR EnsemblProtists; EAL71907; EAL71907; DDB_G0269114.
DR EnsemblProtists; EEU04155; EEU04155; DDB_G0269114.
DR GeneID; 8617781; -.
DR KEGG; ddi:DDB_G0269114; -.
DR dictyBase; DDB_G0269114; numA.
DR eggNOG; ENOG502RFA6; Eukaryota.
DR HOGENOM; CLU_368610_0_0_1; -.
DR InParanoid; Q9U7C9; -.
DR OMA; EYVHYCA; -.
DR PRO; PR:Q9U7C9; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005813; C:centrosome; IDA:dictyBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:dictyBase.
DR GO; GO:0005730; C:nucleolus; IDA:dictyBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:dictyBase.
DR GO; GO:0048188; C:Set1C/COMPASS complex; IBA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IPI:dictyBase.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; IMP:dictyBase.
DR Gene3D; 3.40.50.10190; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR036420; BRCT_dom_sf.
DR Pfam; PF00533; BRCT; 1.
DR SMART; SM00292; BRCT; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF52113; SSF52113; 1.
DR PROSITE; PS50172; BRCT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Calmodulin-binding; Nucleus;
KW Reference proteome; Repeat.
FT CHAIN 1..756
FT /note="Nucleomorphin"
FT /id="PRO_0000293632"
FT DOMAIN 124..216
FT /note="BRCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT REGION 1..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 272..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..512
FT /note="Calmodulin binding"
FT /evidence="ECO:0000269|PubMed:11919178"
FT REGION 514..587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 537..588
FT /note="DEED region"
FT /evidence="ECO:0000269|PubMed:11919178"
FT REGION 589..606
FT /note="Calmodulin binding"
FT /evidence="ECO:0000269|PubMed:11919178"
FT REGION 596..613
FT /note="Calmodulin binding"
FT /evidence="ECO:0000269|PubMed:11919178"
FT REGION 613..639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 660..700
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 464..480
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:15896312"
FT COMPBIAS 284..298
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 436..450
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..529
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 536..586
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..254
FT /note="Missing (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:11919178"
FT /id="VSP_052481"
SQ SEQUENCE 756 AA; 85404 MW; C5DF0DB1A281C836 CRC64;
MDLDYSSDNS RLVADNQNNK TNTKNQSQLS TSSQIPQTSQ SYQQQQQHNQ QQQQQQQQKQ
NSRPSSPKTI QAKVNNNNNN NNNNNNNNNN NNNNGATISH PPTSQSNEDL SSSAEIHHDD
SAQLNSNIFE NLGFVIDITI NLKRKMELVK IIKKNGGKSC YSCTKATHLI TTKQGYLEKT
QKVTQAISLG IPILVKEYVH YCAFKKELLG VENYLVSSLI LNPTSTTLNS SQDLIEKPNE
EEPQNGTINE QPIAMTIDNT LESNKPIETI QPSSLEKQEP KETELQTQQQ PQTELKSELE
PQQLQLIQEK DKNINNLSID SNHMDTSKIE AGNNISNQTQ SNIISSVVQT IEPLKECNKI
DLDNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNSSSNNKN KNNVDEAQEG LNINSVMDVH
LTSSTSSTLS SSDNQDNELL RDEGTTPKSK KKFSQKKNHL LNLKKSYQDP EIIAHSRPRK
SSGGVSLVEA LSDHANYISN LDGFKYYARA NKSSLNSNAT TSGGNNRSIK LNEYKYDDEE
EDEEDEDEED EEEDEEEEEE EEEEEEDYDD EDLNDEESDE DDFDSDEDVS RFIKGKLLQK
QQKIYKDLER FEHSRQQQHH HHQSSQVNSS KPRSRSHSRD YIESEIAKYL LNNSEKQIPL
SPTKKRSLSN QFHQDGGNNT TDGNLFNNFS TNGGGNRTNI DDSIKQSILE SPSSSSLKSS
KSKKSKKPFL PPLSTLVHIL VTFIILSAFF MSLPSN
