ID   NUCS1_HALSA             Reviewed;         240 AA.
AC   Q9HSL7;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Endonuclease NucS 1 {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS1 {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=VNG_0171C;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AE004437; AAG18787.1; -; Genomic_DNA.
DR   PIR; G84177; G84177.
DR   RefSeq; WP_010902082.1; NC_002607.1.
DR   AlphaFoldDB; Q9HSL7; -.
DR   SMR; Q9HSL7; -.
DR   STRING; 64091.VNG_0171C; -.
DR   PaxDb; Q9HSL7; -.
DR   EnsemblBacteria; AAG18787; AAG18787; VNG_0171C.
DR   GeneID; 5952407; -.
DR   KEGG; hal:VNG_0171C; -.
DR   PATRIC; fig|64091.14.peg.123; -.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   InParanoid; Q9HSL7; -.
DR   OMA; VHTDEGQ; -.
DR   OrthoDB; 96363at2157; -.
DR   PhylomeDB; Q9HSL7; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR010916; TonB_box_CS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..240
FT                   /note="Endonuclease NucS 1"
FT                   /id="PRO_0000155689"
SQ   SEQUENCE   240 AA;  24602 MW;  5461ED0E86E84471 CRC64;
     MPRTLTDPTA DRAAAFATDA AADGLTVSLI GSCVATLTGR SQRDLAAGVR TLLWKPDDTV
     VVHGASGRDP DAWASGGPVT VDAADGLTVA CDGGHTAGAL RVRFDAVHTA TAFDAAGADA
     TTVSGTEAAL KDRVLDTPDL VEPGFQPLAT ERDTPAGPID IYGRDADGTV TAVELKNVRA
     GPAAASQLQR YVAALRRTLH ADATVRGILV APAVTAKTRR LLADRGLTFS PVSPPGSRDR