ID   NUCS2_HALSA             Reviewed;         250 AA.
AC   Q9HNR1;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Endonuclease NucS 2 {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS2 {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=VNG_1986C;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004437; AAG20159.1; -; Genomic_DNA.
DR   PIR; C84349; C84349.
DR   RefSeq; WP_010903460.1; NC_002607.1.
DR   AlphaFoldDB; Q9HNR1; -.
DR   SMR; Q9HNR1; -.
DR   STRING; 64091.VNG_1986C; -.
DR   PaxDb; Q9HNR1; -.
DR   EnsemblBacteria; AAG20159; AAG20159; VNG_1986C.
DR   GeneID; 5954017; -.
DR   GeneID; 62887317; -.
DR   KEGG; hal:VNG_1986C; -.
DR   PATRIC; fig|64091.14.peg.1516; -.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   InParanoid; Q9HNR1; -.
DR   OMA; RCSVDYA; -.
DR   OrthoDB; 96363at2157; -.
DR   PhylomeDB; Q9HNR1; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..250
FT                   /note="Endonuclease NucS 2"
FT                   /id="PRO_0000155690"
SQ   SEQUENCE   250 AA;  27412 MW;  4B11F28F381569E6 CRC64;
     MTVERVSAPT PAAFVDAANA AFRNGAVLSV QARCEVEYNG RTSGYLGDGD RLLVAKPDGT
     FLVHQPTGHK PVNWMPGGGT VEARTSQGDA VLLARRSNPT ERVETRLHEV YGVTRFDAED
     GATYEESGTE AEMHEYIEAN PDALEAGLRI VEHERETKYG FIDFYAVDGD GTPVVIEVKR
     IQATLNHFDQ LKRYVDRYAE TNDDVRGMLV APSASERVTR ALRDNGLEFV ALAEFGLDAK
     GATEAKLTDF