ID   NUCS_AERPE              Reviewed;         261 AA.
AC   Q9YDF6;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=APE_0957;
OS   Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS   K1).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Aeropyrum.
OX   NCBI_TaxID=272557;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX   PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA   Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA   Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT   "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT   Aeropyrum pernix K1.";
RL   DNA Res. 6:83-101(1999).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; BA000002; BAA79941.1; -; Genomic_DNA.
DR   PIR; E72692; E72692.
DR   AlphaFoldDB; Q9YDF6; -.
DR   SMR; Q9YDF6; -.
DR   STRING; 272557.APE_0957; -.
DR   EnsemblBacteria; BAA79941; BAA79941; APE_0957.
DR   KEGG; ape:APE_0957; -.
DR   PATRIC; fig|272557.25.peg.688; -.
DR   eggNOG; arCOG01304; Archaea.
DR   OMA; VHTDEGQ; -.
DR   Proteomes; UP000002518; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..261
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155688"
SQ   SEQUENCE   261 AA;  28928 MW;  F88C3BD7BC9C4DA2 CRC64;
     MSQEASVDGR FGVASSPTIE EAAGLLEKLL DGSSMVVVAG VCSSEYEGRG ASVSTEGDKL
     LIVKPGGAVI LHGPRGFRPL NWQPSTSHTE VATADGLLTL KFYRRTPREV LKIACGSIWY
     IAWVRFPEEG AFWMYMTEDD LRKAVALHPR ELLGEDIRFF AEEKRTPSGK ADLYGVDERG
     NIVIVEVKRV RADESAVRQL EGYVRDYPTQ AKVRGILVAP DISDAARRLL ESRGLEFRRV
     DLKKAYSLLK PGRGRSVLDF L