ID   NUCS_ARTS2              Reviewed;         231 AA.
AC   A0JY58;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=Arth_2599;
OS   Arthrobacter sp. (strain FB24).
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=290399;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FB24;
RX   PubMed=24501649; DOI=10.4056/sigs.4438185;
RA   Nakatsu C.H., Barabote R., Thompson S., Bruce D., Detter C., Brettin T.,
RA   Han C., Beasley F., Chen W., Konopka A., Xie G.;
RT   "Complete genome sequence of Arthrobacter sp. strain FB24.";
RL   Stand. Genomic Sci. 9:106-116(2013).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP000454; ABK03978.1; -; Genomic_DNA.
DR   RefSeq; WP_011692440.1; NC_008541.1.
DR   AlphaFoldDB; A0JY58; -.
DR   SMR; A0JY58; -.
DR   STRING; 290399.Arth_2599; -.
DR   EnsemblBacteria; ABK03978; ABK03978; Arth_2599.
DR   KEGG; art:Arth_2599; -.
DR   eggNOG; COG1637; Bacteria.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   OrthoDB; 1002946at2; -.
DR   Proteomes; UP000000754; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..231
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000045825"
SQ   SEQUENCE   231 AA;  25561 MW;  E8234EECA32C441E CRC64;
     MRLVIARCSV DYVGRLKAHL PLATRLLLVK ADGSVLVHSD GGSYKPLNWM SPPASLRVST
     PDEVDLELGV TEQWTVQSAK TDDRLIINIH EQLHDTSHEL GQDPGLIKDG VEADLQRLLA
     DQIERLGTGF SLIRREYFTA IGPVDILARD ADGATVAIEL KRRGDIDGVE QLTRYLELLN
     RDPLLAPVRG IFAAQQIKPQ AKVLANDRGI DCVTLDYDAM RGVDDSESRL F