ID   NUCS_BIFA0              Reviewed;         234 AA.
AC   B8DWS0;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=BLA_0628;
OS   Bifidobacterium animalis subsp. lactis (strain AD011).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=442563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AD011;
RX   PubMed=19011029; DOI=10.1128/jb.01515-08;
RA   Kim J.F., Jeong H., Yu D.S., Choi S.-H., Hur C.-G., Park M.-S., Yoon S.H.,
RA   Kim D.-W., Ji G.E., Park H.-S., Oh T.K.;
RT   "Genome sequence of the probiotic bacterium Bifidobacterium animalis subsp.
RT   lactis AD011.";
RL   J. Bacteriol. 191:678-679(2009).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP001213; ACL28921.1; -; Genomic_DNA.
DR   RefSeq; WP_004219182.1; NC_011835.1.
DR   AlphaFoldDB; B8DWS0; -.
DR   SMR; B8DWS0; -.
DR   STRING; 442563.BLA_0628; -.
DR   EnsemblBacteria; ACL28921; ACL28921; BLA_0628.
DR   GeneID; 66533759; -.
DR   KEGG; bla:BLA_0628; -.
DR   PATRIC; fig|442563.4.peg.659; -.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000002456; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..234
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000198194"
SQ   SEQUENCE   234 AA;  25928 MW;  05660C2D11891476 CRC64;
     MRIIVADCCA EYTGRLSASL PLAKRVLLIK ADNSLLIFSE IGSYKPLNWM LSPCTIKDIT
     PQDADDDPAT PTPEKIIRVQ STKTNDVLEV TLQHIYSDET HDLGTDPGLR KDGVEDHLQR
     YLAEQIERIG EGAKLVRREY PTAIGPVDIM AIDADGTHVA IEIKRHGGID GVEQLTRYCE
     LLNRDPLLAP VRGIFAAQTI TPQAQVLAKD RGFDCLLLDY DDMKGTDDGA LRLF