ID   NUCS_BIFAA              Reviewed;         234 AA.
AC   A1A3C3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=BAD_1425;
OS   Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 /
OS   E194a).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=367928;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15703 / DSM 20083 / NCTC 11814 / E194a;
RA   Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S.,
RA   Tanaka K., Watanabe K.;
RT   "Bifidobacterium adolescentis complete genome sequence.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AP009256; BAF40206.1; -; Genomic_DNA.
DR   RefSeq; WP_011743716.1; NC_008618.1.
DR   AlphaFoldDB; A1A3C3; -.
DR   SMR; A1A3C3; -.
DR   STRING; 1680.BADO_1606; -.
DR   EnsemblBacteria; BAF40206; BAF40206; BAD_1425.
DR   KEGG; bad:BAD_1425; -.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000008702; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..234
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000045826"
SQ   SEQUENCE   234 AA;  26055 MW;  3A38158DD614D98B CRC64;
     MRIIVADCSA EYSERLNASL PLAKRVLLIK ADNSLLIFSE LGSYKPLNWM SAPCSIRDIT
     PDHADDDVDT EVPQKVIRAS ADKSNDVLEV TLQRIYSDES YDLGEDPGLI KDGVEDHLQK
     YLAEQIERIG KGAKLVRREY PTAIGPVDIM AIDGNGEHVA IEIKRNGGID GVEQLTRYCD
     LLNRDPLLAP VHGIFAAQTI TPQARVLAQD RGFKCLLLDY EEMKGTEDDS LRLF