ID   NUCS_BIFLD              Reviewed;         256 AA.
AC   B3DTU8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=BLD_1121;
OS   Bifidobacterium longum (strain DJO10A).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=205913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DJO10A;
RX   PubMed=18505588; DOI=10.1186/1471-2164-9-247;
RA   Lee J.H., Karamychev V.N., Kozyavkin S.A., Mills D., Pavlov A.R.,
RA   Pavlova N.V., Polouchine N.N., Richardson P.M., Shakhova V.V.,
RA   Slesarev A.I., Weimer B., O'Sullivan D.J.;
RT   "Comparative genomic analysis of the gut bacterium Bifidobacterium longum
RT   reveals loci susceptible to deletion during pure culture growth.";
RL   BMC Genomics 9:247-247(2008).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP000605; ACD98567.1; -; Genomic_DNA.
DR   RefSeq; WP_007051476.1; NZ_AABM02000027.1.
DR   AlphaFoldDB; B3DTU8; -.
DR   SMR; B3DTU8; -.
DR   EnsemblBacteria; ACD98567; ACD98567; BLD_1121.
DR   GeneID; 66504483; -.
DR   KEGG; blj:BLD_1121; -.
DR   HOGENOM; CLU_069350_0_1_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000002419; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 2.
DR   Pfam; PF01939; NucS; 2.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..256
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000198195"
FT   REGION          62..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   256 AA;  27867 MW;  428B743E320C7AA0 CRC64;
     MRVIVADCSA EYSGRLNASL PLAKRVLLIK ADSSLLIFSE LGSYKPLNWM TAPCTIREID
     PAAKSAQHSR ESVAGGAVDG DSATHSPESV AAGEPEKVLR VSADKGSDIL EVTLQHIYSD
     QTYDLGEDPG LIKDGVEDHL QRYLAEQIER IGKGAKLVRR EYPTAIGPVD IMAVNAEGEH
     VAVEIKRHGG IDGVEQLTRY CELLNRDPLL APVHGIFAAQ TITPQAQVLA KDRGFTCLIL
     DYDDMKGTED DSLRLF