ID   NUCS_BIFLO              Reviewed;         256 AA.
AC   Q8G7B5;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=BL0355;
OS   Bifidobacterium longum (strain NCC 2705).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=206672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCC 2705;
RX   PubMed=12381787; DOI=10.1073/pnas.212527599;
RA   Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G.,
RA   Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.;
RT   "The genome sequence of Bifidobacterium longum reflects its adaptation to
RT   the human gastrointestinal tract.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN24193.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014295; AAN24193.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_695557.1; NC_004307.2.
DR   RefSeq; WP_007051476.1; NC_004307.2.
DR   AlphaFoldDB; Q8G7B5; -.
DR   SMR; Q8G7B5; -.
DR   STRING; 206672.BL0355; -.
DR   EnsemblBacteria; AAN24193; AAN24193; BL0355.
DR   GeneID; 66504483; -.
DR   KEGG; blo:BL0355; -.
DR   PATRIC; fig|206672.9.peg.1091; -.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000000439; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 2.
DR   Pfam; PF01939; NucS; 2.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..256
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155680"
FT   REGION          62..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   256 AA;  27867 MW;  428B743E320C7AA0 CRC64;
     MRVIVADCSA EYSGRLNASL PLAKRVLLIK ADSSLLIFSE LGSYKPLNWM TAPCTIREID
     PAAKSAQHSR ESVAGGAVDG DSATHSPESV AAGEPEKVLR VSADKGSDIL EVTLQHIYSD
     QTYDLGEDPG LIKDGVEDHL QRYLAEQIER IGKGAKLVRR EYPTAIGPVD IMAVNAEGEH
     VAVEIKRHGG IDGVEQLTRY CELLNRDPLL APVHGIFAAQ TITPQAQVLA KDRGFTCLIL
     DYDDMKGTED DSLRLF